From red to green: the propidium iodide-permeable membrane of Shewanella decolorationis S12 is repairable.
From red to green: the propidium iodide-permeable membrane of Shewanella decolorationis S12 is repairable.
复制标题
从红到绿：脱色希瓦氏菌S12的碘化丙啶渗透膜是可修复的
DOI：
10.1038/srep18583
复制
发表时间：
2015-12-21
影响因子：
4.6
通讯作者：
Xu M
中科院分区：
文献类型：
--
作者：
Yang Y;Xiang Y;Xu M
活力是几乎所有微生物过程中共同关注的问题。基于生物活性的测定广泛用于微生物活力评估，特别是用于混合物种样品或生物膜。碘化丙啶（PI）是最常用的基于膜渗透性的细胞活力的荧光指示剂。我们的研究结果表明，富马酸呼吸琥珀酸的积累可以诱导脱色希瓦氏菌生物膜细胞的PI渗透。共聚焦激光扫描显微镜进一步显示，PI渗透膜可以修复原位时，细胞外琥珀酸通过切换延胡索酸呼吸电极呼吸消除。在膜修复的同时，原始PI渗透性细胞的电极呼吸能力恢复。琼脂集落计数表明，修复的细胞的主要部分是活的，但nonculturable（VBNC）。结果表明，S.脱色S12具有修复PI渗透膜的能力，这表明重新评估PI渗透细菌的命运和功能，并扩大了我们对恶劣环境中细菌存活状态灵活性的认识。
Viability is a common issue of concern in almost all microbial processes. Fluorescence-based assays are extensively used in microbial viability assessment, especially for mixed-species samples or biofilms. Propidium iodide (PI) is the most frequently used fluorescence indicator for cell viability based on the membrane permeability. Our results showed that the accumulation of succinate from fumarate respiration could induce PI-permeability in Shewanella decolorationis biofilm cells. Confocal laser scanning microscope further showed that the PI-permeable membrane could be repaired in situ when the extracellular succinate was eliminated by switching fumarate respiration to electrode respiration. Simultaneously with the membrane repair, the electrode respiring capacity of the originally PI-permeable cells was recovered. Agar-colony counts suggested that a major portion of the repaired cells were viable but nonculturable (VBNC). The results evidenced that S. decolorationis S12 has the capacity to repair PI-permeable membranes which suggests a reevaluation of the fate and function of the PI-permeable bacteria and expanded our knowledge on the flexibility of bacterial survival status in harsh environments.