A maize homolog of mammalian CENPC is a constitutive component of the inner kinetochore
A maize homolog of mammalian CENPC is a constitutive component of the inner kinetochore
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DOI：
10.1105/tpc.11.7.1227
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发表时间：
1999-07-01
期刊：
影响因子：
11.6
通讯作者：
Hiatt, EN
中科院分区：
文献类型：
--
作者：
Dawe, RK;Reed, LM;Hiatt, EN
已经鉴定了被称为着丝粒蛋白C（CENPC）的保守动粒组装蛋白的三种玉米同源物（CenpcA、CenpcB和CenpcC）的基因。玉米CENPC的C-末端部分与哺乳动物CENPC及其酵母同系物Mif 2 p在称为区域I的23个氨基酸区域上具有相似性。结合三维右显微镜的免疫定位实验表明，CENPC是整个间期，有丝分裂和减数分裂的动粒的组成部分。结果表明，在减数分裂过程中，姐妹动粒分离发生在两个不连续的阶段。姐妹动粒的部分分离发生在前中期I，完全分离发生在前中期II。CENPC不存在于称为新着丝粒的结构上，在玉米中，新着丝粒表现出向极运动，但缺乏着丝粒/动粒的其他重要特征。CENPC和先前鉴定的着丝粒DNA序列密切相互作用，但不严格共定位在减数分裂染色体上。这些和其他数据表明，CENPC占据玉米动粒的内部域。
Genes for three maize homologs (CenpcA, CenpcB, and CenpcC) of the conserved kinetochore assembly protein known as centromere protein C (CENPC) have been identified. The C-terminal portion of maize CENPC shares similarity with mammalian CENPC and its yeast homolog Mif2p over a 23-amino acid region known as region I. Immunolocalization experiments combined with three-dimensional right microscopy demonstrated that CENPC is a component of the kinetochore throughout interphase, mitosis, and meiosis. It is shown that sister kinetochore separation occurs in two discrete phases during meiosis. A partial separation of sister kinetochores occurs in prometaphase I, and a complete separation occurs in prometaphase II. CENPC is absent on structures known as neocentromeres that, in maize, demonstrate poleward movement but lack other important features of centromeres/kinetochores. CENPC and a previously identified centromeric DNA sequence interact closely but do not strictly colocalize on meiotic chromosomes. These and other data indicate that CENPC occupies an inner domain of the maize kinetochore.