Genetic and serological identification of three Vibrio parahaemolyticus strains as candidates for novel provisional O serotypes
Genetic and serological identification of three Vibrio parahaemolyticus strains as candidates for novel provisional O serotypes
复制标题
三种副溶血弧菌菌株作为新型临时 O 血清型候选者的遗传和血清学鉴定
DOI：
10.1016/j.ijfoodmicro.2017.01.010
复制
发表时间：
2017-03-20
影响因子：
5.4
通讯作者：
Feng, Lu
中科院分区：
文献类型：
--
作者：
Guo, Xi;Liu, Bin;Feng, Lu
Vibrio parahaemolyticus is a Gram-negative, halophilic Vibrio that naturally inhabits marine and estuarine environments worldwide and has recently been recognized as one of the most important foodborne pathogens. To date, 13 O serotypes and 71 K serotypes of V. parahaemolyticus have been identified. However, untypeable V. parahaemolyticus strains are frequently found during routine detection, indicating that other forms of serotypes exist and suggesting the necessity for extension of the antigenic scheme. In this work, through the genetic analysis of the O serotype genetic determinants (OGDs) and the production of antisera and serological tests, we identified three novel O serotypes of V. parahaemolyticus. Rirther analyses showed that recombination and gene-set deletions/insertions within OGDs may play key roles id the generation of V. parahaemolyticus O serotype diversity. A PCR method was developed for the identification of these novel O serotypes, and specificity and sensitivity were evaluated. A double-blind test including 283 clinical isolates was performed, giving perfect coitelation with the agglutination test results. Generally, our study expanded the O-antigenic scheme of V. parahaerholyticus from 13 to 16 and provided a tool with the potential for the detection and identification of V. parahaemolyticus strains (especially untypeable strains) isolated from both the clinic and the environment. (C) 2017 Elsevier B.V. All rights reserved.
