Highly fluorescent carbon dots as selective and visual probes for sensing copper ions in living cells via an electron transfer process
Highly fluorescent carbon dots as selective and visual probes for sensing copper ions in living cells via an electron transfer process
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高荧光碳点作为选择性和视觉探针，用于通过电子转移过程感测活细胞中的铜离子
DOI：
10.1016/j.bios.2017.05.035
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发表时间：
2017-11-15
影响因子：
12.6
通讯作者：
Huang, Cheng Zhi
中科院分区：
文献类型：
--
作者：
Wang, Jian;Li, Rong Sheng;Huang, Cheng Zhi
Cu2+作为许多重要酶的组成部分，参与了许多重要的生物过程，当Cu2+过量时，这些过程与氧化损伤和环境污染有关。在这项工作中，Cu2+可以被碳点（CDs）的氨基捕获形成配合物，通过非辐射电子转移过程导致CDs的强荧光猝灭，这为Cu2+检测提供了一种快速，直观和选择性的方法。该探针对Cu2+具有较宽的响应浓度范围（0.01 ~ 2 μ M），检出限为6.7 nM。值得注意的是，CDs具有良好的生物相容性和光稳定性，适用于Cu2+动态侵入活细胞和摩桑罗非鱼的可视化。此外，镉可以通过形成络合物来抑制Cu2+离子对活细胞的毒性。
As an integral part of many important enzymes, Cu2+ is involved in a number of vital biological processes, which is linked to the oxidative damage and environmental contamination when Cu2+ is excessive. In this work, Cu2+ can be captured by the amino groups of carbon dots (CDs) to form complexes, resulting in a strong fluorescence quenching of CDs via a nonradiative electron transfer process, which offered a rapid, visual, and selective methodology for Cu2+ detection. The probe exhibited a wide response concentration range (0.01-2 mu M) to Cu2+ with a detection limit of 6.7 nM. Significantly, the CDs presented excellent biocompatibility and high photostability, which were applicable for the visualization of Cu2+ dynamic invasion into living cells and Tilapia mossambica. Furthermore, the toxicity of Cu2+ ions to living cells could be inhibited with CDs by the formation of complexes.