Anti-obesity Effect of Capsaicin in Mice Fed with High-Fat Diet Is Associated with an Increase in Population of the Gut Bacterium Akkermansia muciniphila.
Anti-obesity Effect of Capsaicin in Mice Fed with High-Fat Diet Is Associated with an Increase in Population of the Gut Bacterium Akkermansia muciniphila.
复制标题
辣椒素对高脂饮食小鼠的抗肥胖作用与肠道细菌阿克曼氏菌数量的增加有关
DOI：
10.3389/fmicb.2017.00272
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发表时间：
2017
影响因子：
5.2
通讯作者：
Le S
中科院分区：
文献类型：
--
作者：
Shen W;Shen M;Zhao X;Zhu H;Yang Y;Lu S;Tan Y;Li G;Li M;Wang J;Hu F;Le S
辣椒素 (CAP) 主要通过激活瞬时受体电位香草酸 1 (TRPV1) 阳离子通道来减轻体重。然而，最近的证据表明，肠道微生物群影响宿主的许多生理过程，并可能引发肥胖。本研究确定了 CAP 的抗肥胖作用是否与肠道微生物群的变化有关。 C57BL/6 小鼠用高脂饮食 (HFD) 或 HFD 加 CAP (HFD-CAP) 喂养 9 周。我们观察到，与 HFD 喂养的小鼠相比，HFD-CAP 喂养的小鼠体重增加显着减少，葡萄糖耐量改善。 16S rRNA 基因测序结果显示，HFD-CAP 喂养的小鼠中变形菌门减少。此外，HFD-CAP 喂养的小鼠表现出更高丰度的 Akkermansia muciniphila，这是一种对宿主代谢具有有益影响的粘蛋白降解细菌。进一步研究发现CAP直接上调肠道粘蛋白2基因Muc2和抗菌蛋白基因Reg3g的表达。这些数据表明 CAP 的抗肥胖作用与适度调节肠道微生物群有关。
Capsaicin (CAP) reduces body weight mainly through activation of transient receptor potential vanilloid 1 (TRPV1) cation channel. However, recent evidence indicates that the gut microbiota influences many physiological processes in host and might provoke obesity. This study determined whether the anti-obesity effect of CAP is related to the changes in gut microbiota. C57BL/6 mice were fed either with high-fat diet (HFD) or HFD with CAP (HFD-CAP) for 9 weeks. We observed a significantly reduced weight gain and improved glucose tolerance in HFD-CAP-fed mice compared with HFD-fed mice. 16S rRNA gene sequencing results showed a decrease of phylum Proteobacteria in HFD-CAP-fed mice. In addition, HFD-CAP-fed mice showed a higher abundance of Akkermansia muciniphila, a mucin-degrading bacterium with beneficial effects on host metabolism. Further studies found that CAP directly up-regulates the expression of Mucin 2 gene Muc2 and antimicrobial protein gene Reg3g in the intestine. These data suggest that the anti-obesity effect of CAP is associated with a modest modulation of the gut microbiota.