THE PRIMARY ROLE OF LYMPHORETICULAR CELLS IN THE MEDIATION OF HOST RESPONSES TO BACTERIAL-ENDOTOXIN
THE PRIMARY ROLE OF LYMPHORETICULAR CELLS IN THE MEDIATION OF HOST RESPONSES TO BACTERIAL-ENDOTOXIN
复制标题
DOI：
10.1093/infdis/141.1.55
复制
发表时间：
1980-01-01
影响因子：
6.4
通讯作者：
MERGENHAGEN, SE
中科院分区：
文献类型：
--
作者：
MICHALEK, SM;MOORE, RN;MERGENHAGEN, SE
通过过继转移脂多糖敏感小鼠的骨髓细胞(C3H/HEJ株)，可使对脂多糖无反应的小鼠(C3H/HEJ株)对脂多糖敏感。采用过继转移模型评价淋巴网状细胞对内毒素对宿主的免疫原性、佐剂效应、致死性、诱生干扰素和诱导集落刺激因子等5种效应的影响。过继移植C3H/HEN小鼠的骨髓细胞后，C3H/HeJ小鼠对上述每一种效应都变得敏感。转移效率与X射线照射剂量成正比，与存活的宿主干细胞数量成反比。最有效辐射剂量为850rad，C3Hfwdarw。在此剂量下制备的C3H/HeJx嵌合体对每一测试参数的敏感性与C3H/HEN供体一样敏感，但对致死性的抵抗力是内毒素反应性C3H/HEN小鼠的3倍。过继转移C3H/HeJ骨髓细胞也可使C3H/HEN小鼠对脂多糖无反应。C3H/HEN嵌合体对所研究的内毒素的所有作用都具有抵抗力，但对脑脊液的诱导作用除外。淋巴细胞和/或巨噬细胞在介导多种不同且看似无关的宿主对内毒素的反应中起主要作用。
Mice unresponsive to lipopolysaccharide (LPS) (strain C3H/HeJ) can be rendered LPS-sensitive by the adoptive transfer of bone marrow cells from LPS-sensitive mice (strain C3H/HeN). This model of adoptive transfer was used to evaluate the contribution of lymphoreticular cells to 5 effects of [Escherichia coli] endotoxin on the host: immunogenicity, adjuvanticity, lethality, inducation of interferon and induction of colony-stimulating factor [CSF]. C3H/HeJ mice became sensitive to each of these effects after adoptive transfer of bone marrow cells from C3H/HeN mice. The efficacy of transfer was directly proportional to the dose of X-irradiation and inversely proportional to the number of surviving host stem cells. The most effective dose of radiation was 850 rad, and C3H/HeN .fwdarw. C3H/HeJx chimeras prepared at this dose were as sensitive to LPS for each parameter tested as were the C3H/HeN donors except for a 3-fold greater resistance to lethality than LPS-responsive C3H/HeN mice. C3H/HeN mice could also be rendered unresponsive to LPS by the adoptive transfer of C3H/HeJ bone marrow cells. C3H/HeN chimeras were resistant to all of the effects of LPS studied except for the induction of CSF. Lymphocytes and/or macrophages play a primary role in mediating a number of diverse and seemingly unrelated host responses to endotoxin.