Cloning, characterization, and heterologous expression of a functional geranylgeranyl pyrophosphate synthase from sunflower (Helianthus annuus L.)
Cloning, characterization, and heterologous expression of a functional geranylgeranyl pyrophosphate synthase from sunflower (Helianthus annuus L.)
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DOI：
10.1016/s0176-1617(00)80109-1
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发表时间：
2000-11-01
影响因子：
4.3
通讯作者：
Han, KH
中科院分区：
文献类型：
--
作者：
Oh, SK;Kim, IJ;Han, KH
香叶基香叶基焦磷酸合成酶（GGPP）是萜类化合物生物合成的关键酶，萜类化合物在植物生长发育和生物体间相互作用中起着重要作用。我们已经克隆并鉴定了向日葵GGPP合酶的cDNA编码。序列分析表明，该基因包含一个1071 bp的开放阅读框，编码一个356个氨基酸残基的多肽，分子量为38.7 kDa。该向日葵GGPS的预测氨基酸序列与其它植物GGPP酶具有高相似性（63- 79%）。利用重组蛋白的体外活性测定和遗传互补实验表明，我们克隆的cDNA编码功能性GGPP合酶。使用向日葵幼苗的基因表达研究表明，该基因在种子吸胀两天后表达。脱落酸处理下调该基因的表达。
Geranylgeranyl pyrophosphate (GGPP) synthase is a key enzyme for the biosynthesis of terpenoid compounds that play vital roles in plant growth and development, and interactions between organisms. We have cloned and characterized a sunflower cDNA encoding GGPP synthase. Sequence analysis showed that the gene contained a 1071-bp open reading frame coding for a peptide of 356 amino acid residues with a calculated molecular mass of 38.7 kDa. The predicted amino acid sequence of this sunflower GGPS has high similarity to other plant GGPP synthases (63-79 %). In vitro activity assay using recombinant protein and genetic complementation experiments have shown that the cDNA we cloned encodes for functional GGPP synthase. Gene expression studies using sunflower seedlings showed that the gene was expressed after two days of seed imbibition. Abscisic acid treatment down-regulated the expression of the gene.