Plasma β-globin DNA as a prognostic marker in chest pain patients
Plasma β-globin DNA as a prognostic marker in chest pain patients
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DOI：
10.1016/j.cca.2005.12.021
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发表时间：
2006-06-01
影响因子：
5
通讯作者：
Lo, Y. M. Dennis
中科院分区：
文献类型：
--
作者：
Rainer, Timothy H.;Lam, Nicole Y. L.;Lo, Y. M. Dennis
背景：急性冠脉综合征可能涉及细胞死亡和核酸释放到循环中。因此，我们研究了血浆DNA浓度是否升高，并确定其在ACS患者中的预后意义。方法：采用实时聚合酶链反应定量测定可能是心源性胸痛的急诊科患者的血液样本中的-珠蛋白基因。结果：分析了58例胸痛患者和21例年龄和性别匹配的健康对照者的样本。与对照组相比，心脏轻度损伤患者的中位血浆DNA浓度升高了1.5倍，STEA和STEMI患者的中位血浆DNA浓度进一步升高，2年内死亡患者的中位血浆DNA浓度最高（P=0.0005；后hoc Dunn's, P < 0.05）。中位血浆DNA浓度在后来发生心力衰竭的患者中较高（1060比500 kGE/l， P=0.0095）；后来再梗死的患者更高（1000 vs 530 kGE/l； P=0.0298）；在该入院期间发生心脏骤停的患者更高（1350 vs 525 kGE/I； P=0.04）；在出院6个月内再次入院的患者中更高（725 vs 475 kGE/I； P=0.04）。结论：血浆DNA是acs后并发症的潜在标志物。(c) 2005 Elsevier B.V.版权所有
Background: Acute coronary syndrome may involve cell death and the release of nucleic acids into the circulation. We thus investigated whether plasma DNA concentrations are increased and determined its prognostic significance in patients with ACS.Methods: Real-time polymerase chain reaction was used to quantitatively measure the beta-globin gene from blood samples taken from patients presenting to an emergency department with chest pain of probable cardiac cause.Results: Samples from 58 patients with chest pain, and from 21 age- and sex-matcbed healthy control subjects were analysed. Compared with the control group, median plasma DNA concentrations were increased 1.5-fold in patients with minor cardiac injury, were increased further in patients with STEA and STEMI, and were the highest in those patients who died within 2 years (P=0.0005; post-hoc Dunn's, P < 0,05). Median plasma DNA concentrations were higher in patients who later developed heart failure (1060 vs. 500 kGE/l; P=0.0095); higher in patients who later reinfarcted (1000 vs. 530 kGE/l; P=0.0298); higher in patients who had a cardiac arrest in that admission (1350 vs. 525 kGE/I; P=0.04); and were higher in patients who were readmitted within 6 months of discharge (725 vs. 475 kGE/I; P=0.04).Conclusion: Plasma DNA is a potential marker for post-ACS complications. (c) 2005 Elsevier B.V. All rights reserved.