Isolation and genetic characterization of cell-lineage mutants of the nematode Caenorhabditis elegans.
Isolation and genetic characterization of cell-lineage mutants of the nematode Caenorhabditis elegans.
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DOI：
10.1093/genetics/96.2.435
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发表时间：
1980-10
期刊：
影响因子：
3.3
通讯作者：
H. Horvitz;J. Sulston
中科院分区：
文献类型：
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作者：
H. Horvitz;J. Sulston
24个突变体改变了秀丽隐杆线虫通常不变的胚胎后细胞系，并进行了分离和遗传表征。在其中一些突变体中，细胞分裂失败，这在野生型动物中发生；在其他突变体中，细胞分裂的方式通常是不一样的。突变体在其缺陷的特异性上有所不同，因此有可能确定影响某些细胞系而不影响其他细胞系的突变。这些突变体定义了14个互补基团，它们已经被绘制出来。大多数细胞系突变的异常表型是由单隐性突变引起的；然而，一种菌株CB1322的过度细胞分裂特征需要存在两个不相关的隐性突变。所有24个细胞系突变体显示不完全外显性和/或可变表达性。其中三个突变体被多效性抑制子抑制，这些多效性抑制子被认为是对零等位基因的特异性，这表明它们的表型是由基因活性完全缺失造成的。
Twenty-four mutants that alter the normally invariant post-embryonic cell lineages of the nematode Caenorhabditis elegans have been isolated and genetically characterized. In some of these mutants, cell divisions fail that occur in wild-type animals; in other mutants, cells divide that do not normally do so. The mutants differ in the specificities of their defects, so that it is possible to identify mutations that affect some cell lineages but not others. These mutants define 14 complementation groups, which have been mapped. The abnormal phenotype of most of the cell-lineage mutants results from a single recessive mutation; however, the excessive cell divisions characteristic of one strain, CB1322, require the presence of two unlinked recessive mutations. All 24 cell-lineage mutants display incomplete penetrance and/or variable expressivity. Three of the mutants are suppressed by pleiotropic suppressors believed to be specific for null alleles, suggesting that their phenotypes result from the complete absence of gene activity.