Identification of a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane.
Identification of a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane.
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酵母线粒体内膜蛋白质输入位点的 45 kDa 蛋白质的鉴定。
DOI：
10.1073/pnas.89.24.11930
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发表时间：
1992
影响因子：
11.1
通讯作者：
Horst,M
中科院分区：
文献类型：
--
作者：
Scherer,PE;Manning-Krieg,UC;Jenö,P;Schatz,G;Horst,M
蛋白质进入线粒体涉及到外膜和内膜的蛋白质转运系统的协作。我们已经在酵母线粒体内膜的蛋白输入部位鉴定出一个45 kDa的蛋白。这个45 kDa的蛋白质可以与部分易位的前体发生交联，当基质中的ATP耗尽时，该前体不能通过内膜输入。此外，一种针对该蛋白的抗体强烈地阻止蛋白质进入右侧向外的内膜囊泡。这种45 kDa的蛋白质只占线粒体蛋白质的0.1%，看起来像是外围附着在内膜的外表面。该蛋白的性质表明它是线粒体内膜蛋白输入系统的一个组成部分。
Import of proteins into mitochondria involves the cooperation of protein translocation systems in the outer and inner membranes. We have identified a 45-kDa protein at the protein import site of the yeast mitochondrial inner membrane. This 45-kDa protein could be crosslinked to a partly translocated precursor, which cannot be imported across the inner membrane when the matrix is depleted of ATP. In addition, an antibody against this protein strongly inhibited protein import into right-side-out inner-membrane vesicles. The 45-kDa protein accounts for only 0.1% of mitochondrial protein and appears peripherally attached to the outer face of the inner membrane. The properties of this protein suggest that it is a component of the protein import system of the mitochondrial inner membrane.