Immunocytochemical localization of secretogranin III in the endocrine pancreas of male rats.
Immunocytochemical localization of secretogranin III in the endocrine pancreas of male rats.
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DOI：
10.1679/aohc.67.57
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发表时间：
2004-03
影响因子：
--
通讯作者：
Y. Sakai;M. Hosaka;A. Yoshinaga;Y. Hira;T. Harumi;Tsuyoshi Watanabe
中科院分区：
文献类型：
--
作者：
Y. Sakai;M. Hosaka;A. Yoshinaga;Y. Hira;T. Harumi;Tsuyoshi Watanabe
分泌颗粒蛋白III（Secretogranin III，Sg III）是颗粒蛋白家族的成员，在神经细胞和内分泌细胞中特异性表达。为了研究内分泌胰腺中SgIII的精确定位，用抗大鼠SgIII的多克隆抗血清对大鼠胰腺组织进行免疫细胞化学分析。通过光学显微镜的半薄切片，免疫反应性的SgIII很容易检测到胰腺A-和B-细胞，微弱的D-细胞，而不是在所有的外分泌胰腺。通过免疫电子显微镜，免疫金颗粒指示的SgIII中观察到的周边区域的分泌颗粒，并普遍在胰腺内分泌细胞。形态计量学分析表明，SgIII是最优先定位于颗粒之间的分泌颗粒的周边。这些发现表明，Sg III与分泌颗粒膜密切相关，用于将其他可溶性成分的聚集体锚在膜上。
Secretogranin III (SgIII), a member of the granin protein family, is expressed specifically in neuronal and endocrine cells. To examine the precise localization of SgIII in the endocrine pancreas, pancreatic tissues of rats were analyzed immunocytochemically with a polyclonal anti-serum raised against rat SgIII. By light microscopy of semithin sections, the immunoreactivity for SgIII was readily detected in pancreatic A- and B-cells, faintly so in D-cells, and not at all in the exocrine pancreas. By immunoelectron microscopy, immunogold particles indicative of SgIII were observed in the peripheral regions of secretory granules, and universally in the pancreatic endocrine cells. Morphometrical analyses indicated that SgIII is most preferentially localized in the periphery of the secretory granule among granins. These findings suggest that SgIII is closely associated with the secretory granule membrane, serving to anchor the aggregates of other soluble constituents to the membrane.