GABAB receptor expression and function in olfactory receptor neuron axon growth.
GABAB receptor expression and function in olfactory receptor neuron axon growth.
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GABAB 受体在嗅觉受体神经元轴突生长中的表达和功能。
DOI：
10.1002/neu.20011
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Puche,AdamC
中科院分区：
文献类型：
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作者：
Priest,CatherineA;Puche,AdamC
神经递质在调节发育中的神经系统的生长锥运动和引导方面起着重要作用。解剖学和电生理学研究表明，成体嗅觉感受器(ORN)神经末梢上存在功能性的GABA受体。使用抗GABABR1a/b受体亚型的抗血清，我们发现发育中的小鼠嗅觉感受器神经元从胚胎第14天到成年期都表达GABA受体。分离的Orns和嗅觉上皮外植体的轴突生长锥体上均存在GABA受体。从胎龄16岁到成年期，嗅球中的神经元开始表达谷氨酸脱羧酶(GAD)，谷氨酸脱羧酶是合成GABA的酶。分离的ONE在GABA受体激动剂巴氯芬或SKF97541存在下培养时，突起生长显著减少。同时给予巴氯芬和GABA受体拮抗剂CGP54626可阻断巴氯芬对ORN突起生长的抑制作用。这些结果表明，生长中的ORN轴突表达GABA受体，并对GABA受体激活的影响敏感。因此，ORNsin vivomomin可以在肾小球旁细胞进入肾小球层时检测到它们释放的GABA，并将其作为限制其生长的信号，并在再生和发育过程中寻找突触靶点。©2004威利期刊，Inc.神经生物学杂志60：154-165,2004
Neurotransmitters have been implicated in regulating growth cone motility and guidance in the developing nervous system. Anatomical and electrophysiological studies show the presence of functional GABABreceptors on adult olfactory receptor neuron (ORN) nerve terminals. Using antisera against the GABABR1a/b receptor isoforms we show that developing mouse olfactory receptor neurons express GABABreceptors from embryonic day 14 through to adulthood. GABABreceptors are present on axon growth cones from both dissociated ORNs and olfactory epithelial explants. Neurons in the olfactory bulb begin to express glutamic acid decarboxylase (GAD), the synthetic enzyme for GABA, from E16 through to adulthood. When dissociated ORNs were cultured in the presence of the GABABreceptor agonists, baclofen or SKF97541, neurite outgrowth was significantly reduced. Concurrent treatment of the neurons with baclofen and the GABABreceptor antagonist CGP54626 prevented the inhibitory effects of baclofen on ORN neurite outgrowth. These results show that growing ORN axons express GABABreceptors and are sensitive to the effects of GABABreceptor activation. Thus, ORNsin vivomay detect GABA release from juxtaglomerular cells as they enter the glomerular layer and use this as a signal to limit their outgrowth and find synaptic targets in regeneration and development. © 2004 Wiley Periodicals, Inc. J Neurobiol 60:154–165, 2004