Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells
Effect of the Ketone Body Beta-Hydroxybutyrate on the Innate Defense Capability of Primary Bovine Mammary Epithelial Cells
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DOI：
10.1371/journal.pone.0157774
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发表时间：
2016-06-16
期刊：
影响因子：
3.7
通讯作者：
Kliem, Heike
中科院分区：
文献类型：
--
作者：
Hillreiner, Maria;Flinspach, Claudia;Kliem, Heike
能量负平衡和酮病被认为会导致奶牛免疫功能受损并增加患临床型乳房炎的风险。本体外研究旨在研究升高水平的主要酮体β-羟基丁酸酯对用乳腺炎病原体大肠杆菌（Escherichia coli，E.大肠杆菌）。因此，从乳汁中分离泌乳中期健康奶牛的pbMEC，并在培养物中用3 mM BHBA和E.杆菌用大肠杆菌刺激pbMEC。coli共刺激6 h或30 h后，pbMEC中多个天然免疫基因表达上调，而3 mM BHBA和E.与单独细菌刺激相比，大肠杆菌刺激导致CCL 2、SAA 3、LF和C3基因表达下调。这些结果表明，增加BHBA浓度可能是部分负责较高的乳房炎易感性的奶牛在泌乳早期。在负能量平衡和酮病期间，血液和乳汁中BHBA水平升高可能通过减弱广泛的先天免疫基因的表达来损害牛乳腺中的先天免疫功能。
Negative energy balance and ketosis are thought to cause impaired immune function and to increase the risk of clinical mastitis in dairy cows. The present in vitro study aimed to investigate the effect of elevated levels of the predominant ketone body beta-hydroxybutyrate on the innate defense capability of primary bovine mammary epithelial cells (pbMEC) challenged with the mastitis pathogen Escherichia coli (E. coli). Therefore, pbMEC of healthy dairy cows in mid-lactation were isolated from milk and challenged in culture with 3 mM BHBA and E. coli. pbMEC stimulated with E. coli for 6 h or 30 h showed an up-regulation of several innate immune genes, whereas co-stimulation of pbMEC with 3 mM BHBA and E. coli resulted in the down-regulation of CCL2, SAA3, LF and C3 gene expression compared to the challenge with solely the bacterial stimulus. These results indicated that increased BHBA concentrations may be partially responsible for the higher mastitis susceptibility of dairy cows in early lactation. Elevated levels of BHBA in blood and milk during negative energy balance and ketosis are likely to impair innate immune function in the bovine mammary gland by attenuating the expression of a broad range of innate immune genes.