A zinc finger protein gene ZFP5 integrates phytohormone signaling to control root hair development in Arabidopsis
A zinc finger protein gene ZFP5 integrates phytohormone signaling to control root hair development in Arabidopsis
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锌指蛋白基因 ZFP5 整合植物激素信号传导来控制拟南芥根毛发育
DOI：
10.1111/j.1365-313x.2012.05094.x
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发表时间：
2012-11-01
期刊：
影响因子：
7.2
通讯作者：
Gan, Yinbo
中科院分区：
文献类型：
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作者：
An, Lijun;Zhou, Zhongjing;Gan, Yinbo
尽管人们对拟南芥根毛的发育进行了广泛的研究，但植物中最大的转录因子家族锌指蛋白是否参与了这一过程仍不清楚。在这里，我们报告的C2H2锌指蛋白锌指蛋白5（ZFP 5）是一个关键的调节器的根毛的起始和形态建成在拟南芥。ZFP 5主要在根中表达，并且优先在根毛细胞中表达。使用zfp5突变体和ZFP5 RNAi株系，我们表明，与野生型相比，ZFP5功能的降低导致根毛更少且更短。遗传和分子实验表明，ZFP 5通过直接促进CAPRICE（CPC）基因的表达来发挥其对根毛发育的影响。此外，我们表明，ZFP 5的表达诱导细胞分裂素，ZFP 5介导细胞分裂素和乙烯对根毛的形成和生长的影响。这些结果表明，ZFP 5整合了多种植物激素信号，以控制拟南芥根表皮细胞的发育。
Although root hair development in Arabidopsis thaliana has been extensively studied, it remains unknown whether the zinc finger proteins, the largest family of transcription factors in plants, are involved in this process. Here we report that the C2H2 zinc finger protein ZINC FINGER PROTEIN 5 (ZFP5) is a key regulator of root hair initiation and morphogenesis in Arabidopsis. ZFP5 is mainly expressed in root and preferentially in root hair cells. Using both zfp5 mutants and ZFP5 RNAi lines, we show that reduction in ZFP5 function leads to fewer and much shorter root hairs compared to wild-type. Genetic and molecular experiments demonstrate that ZFP5 exerts its effect on root hair development by directly promoting expression of the CAPRICE (CPC) gene. Furthermore, we show that ZFP5 expression is induced by cytokinin, and that ZFP5 mediates cytokinin and ethylene effects on the formation and growth of root hairs. These results suggest that ZFP5 integrates various plant hormone cues to control root epidermal cell development in Arabidopsis.