Increased expression of EphA2 and E-N cadherin switch in primary hepatocellular carcinoma
Increased expression of EphA2 and E-N cadherin switch in primary hepatocellular carcinoma
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DOI：
10.1700/1390.15457
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发表时间：
2013-11-01
期刊：
影响因子：
1.9
通讯作者：
Li, Xinying
中科院分区：
文献类型：
--
作者：
Fan, Min;Liu, Yu;Li, Xinying
目的。探讨肝配蛋白A型受体2及上皮间质转化相关蛋白在原发性肝细胞癌中的表达及临床意义。采用免疫化学法检测52例原发性肝细胞癌组织和12例人正常肝组织中肝配蛋白A型受体2、E-钙粘蛋白和N-钙粘蛋白的表达。研究肝细胞癌的临床病理特征和术后肿瘤复发与这些分子表达和E-N钙粘蛋白转换的关系。结果。与正常肝组织相比，肝细胞癌中肝配蛋白A型受体2和N-钙粘蛋白的表达显着增加，而E-钙粘蛋白的表达显着减少。单变量分析显示，不良的临床病理特征与肝配蛋白 A 型受体 2、E-钙粘蛋白、N-钙粘蛋白和 E-N 钙粘蛋白开关的表达密切相关。经过年龄、性别、AFP、HBsAg、肝硬化、肿瘤大小、结节、包膜、门静脉侵犯、细胞分化和 TNM 分期校正后，Ephrin A 型受体 2 和 E-cadherin 表达被证实为独立预后因素。结论。肝配蛋白A型受体2蛋白的过度表达与肿瘤数量、包膜完整性、门静脉癌栓及临床分期相关。肝配蛋白A型受体2调控的上皮间质转化参与侵袭性临床病理特征和预后，提示该受体可能在肝细胞癌的进展和转移中发挥重要作用。
Aim. To investigate the expression and clinical significance of ephrin type-A receptor 2 and epithelial-mesenchymal transition-related proteins in primary hepatocellular carcinoma.Methods. Tissues from 52 primary hepatocellular carcinomas and 12 human normal liver tissues were detected for expression of ephrin type-A receptor 2, E-cadherin, and N-cadherin by immunochemistry. Cinicopathological features of hepatocellular carcinoma and tumor recurrence after operation were studied for the association with these molecular expressions and E-N cadherin switch.Results. Increased expressions of ephrin type-A receptor 2 and N-cadherin and reduced expression of E-cadherin were significantly detected in hepatocellular carcinoma compared with normal liver tissues. Univariate analysis showed that there were close associations between unfavorable clinicopathological features and expressions of ephrin type-A receptor 2, E-cadherin, N-cadherin, and E-N cadherin switch. Ephrin type-A receptor 2 and E-cadherin expressions were confirmed as independent prognostic factors when corrected with age, gender, AFP, HBsAg, liver cirrhosis, tumor size, nodules, capsule, portal vein invasion, cell differentiation, and TNM stage.Conclusions. The overexpression of ephrin type-A receptor 2 protein is correlated with the number of tumors, capsular integrity, portal vein cancer thrombus and clinical stages. Epithelial-mesenchymal transition regulated by ephrin type-A receptor 2 is involved in the aggressive clinicopathological features and prognosis, suggesting that the receptor may play an important role in the progression and metastasis of hepato cellular carcinoma.