DNA synthesis in temperature sensitive mutants of Escherichia coli.
DNA synthesis in temperature sensitive mutants of Escherichia coli.
复制标题
大肠杆菌温度敏感突变体中的 DNA 合成。
DOI：
10.1101/sqb.1968.033.01.036
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
M. Kohiyama
中科院分区：
文献类型：
--
作者：
M. Kohiyama
One of the characteristics of the control mechanism of the DNA replication in Escherichia coli is that the replication proceeds from a fixed locus by a single growing point in a sequential way (Lark, Repkb, and Hoffman, 1963~ Cairns, 1963). This control mechanism may be closely related to the fact that the initiation of DNA replication requires the synthesis of certain proteins (Hanawalt, Maaloe, Cummings, and Schaechter, 1961). One possible function of these proteins might be to catalyze the priming reaction necessary for the action of DNA polymerase (Jacob, Brenner, and Cuzin, 1963). In fact, a temperature sensitive mutant has been isolated whose DNA synthesis at 41~ shows the same kinetics as those of wild type in the absence of protein synthesis (Kohiyama, Lanfrom, Brenner, and Jacob, 1963). Preliminary experiments suggested that the function of the DNA polymerase in crude extracts of this temperature sensitive mutant is normal even at high temperature.This report describes results which show that chromosome replication can occur after~,-ray irradiation even at 41~ in this mutant whose DNA synthesis is otherwise stopped (Kohiyama and Marcovich, 1967). This phenomenon, which is more clearly observed in the mutant than in the wild type, might suggest the participation of some accessory proteins, besides the DNA polymerase, in the control of DNA replication.
