Evaluation of cognitive behaviors in young offspring of C57BL/6J mice after gestational nicotine exposure during different time-windows
Evaluation of cognitive behaviors in young offspring of C57BL/6J mice after gestational nicotine exposure during different time-windows
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DOI：
10.1007/s00213-013-3175-9
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发表时间：
2013-12-01
影响因子：
3.4
通讯作者：
Nabeshima, Toshitaka
中科院分区：
文献类型：
--
作者：
Alkam, Tursun;Kim, Hyoung-Chun;Nabeshima, Toshitaka
妊娠期尼古丁暴露与年轻后代的认知异常有关。然而，由于缺乏对潜在机制的系统研究，目前还没有预防或治疗后代认知行为受损的实用策略。因此，本研究旨在研究妊娠和/或围产期尼古丁暴露（GPNE）对C57BL/6J小鼠后代认知行为的影响，以提供系统的行为数据。怀孕小鼠在六个时间窗口期间通过加糖饮用水接触尼古丁，包括妊娠第 0 天至第 13 天 (G0-G13)、G14-出生后第 0 天 (P0)、G0-P0、G14-P7、G0-P7 和 P0-P7。在 P42-P56 天内，雄性和雌性后代都接受了一系列行为测试。根据暴露时间的不同，GPNE 会不同程度地损害雄性和雌性后代的工作记忆、基于物体的注意力和前脉冲抑制。 G14-P0 期间的尼古丁暴露也会降低 P28 和 P56 前额皮质中去甲肾上腺素的周转率。总体结果表明，在发育的任何时间窗口期间接触尼古丁都会损害后代的认知行为，并建议应选择某些时间窗口（例如 G14-P0）来进一步研究潜在的神经化学或分子机制。
Gestational nicotine exposure is associated with cognitive abnormalities in young offspring. However, practical strategies for prevention or treatment of impaired cognitive behaviors of offspring are not available due to the lack of systematic investigation of underlying mechanism. Therefore, this study aimed at examining the effects of gestational and/or perinatal nicotine exposure (GPNE) on cognitive behaviors in offspring of C57BL/6J mice to provide systematic behavioral data. Pregnant mice were exposed to nicotine via sweetened drinking water during six time-windows, including gestational day 0 to day 13 (G0-G13), G14-postnatal day 0 (P0), G0-P0, G14-P7, G0-P7, and P0-P7. During P42-P56 days, both male and female offspring were given a battery of behavioral tests. Depending on the time of exposure, GPNE impaired working memory, object-based attention, and prepulse inhibition in male and female offspring to different extents. Nicotine exposure during G14-P0 also decreased norepinephrine turnover in the prefrontal cortex on P28 and P56. Overall results indicate that nicotine exposure during any time-windows of development impairs cognitive behaviors in offspring, and suggest that certain time-windows, e.g., G14-P0, should be selected for further studies on the underlying neurochemical or molecular mechanisms.