Patterns of molecular variation in a species-wide germplasm set of Brassica napus
Patterns of molecular variation in a species-wide germplasm set of Brassica napus
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DOI：
10.1007/s00122-011-1676-7
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发表时间：
2011-12-01
影响因子：
5.4
通讯作者：
Stich, Benjamin
中科院分区：
文献类型：
--
作者：
Bus, Anja;Koerber, Niklas;Stich, Benjamin
甘蓝型油菜（Brassica napus L.）是欧洲主要的油料作物，是食用油和可再生能源的来源。我们的研究的目的是（i）检查一个大的和多样化的B的人口结构。（ii）研究不同种质类型内和种质类型间的遗传多样性模式;（iii）比较B和B的基因组。油菜遗传多样性方面，和（iv）简单重复序列（SSR）标记之间的连锁不平衡（LD）的程度进行评估。我们的研究是基于509 B。利用89对SSR引物组合对甘蓝型油菜自交系进行基因分型。主坐标分析和软件结构显示，冬季型，春季型，瑞典人被分配到三个主要的集群。冬油菜的遗传多样性低于其他种质类型。在冬季油菜类型，遗传多样性的衰减与最近的释放日期和减少的芥酸和硫代葡萄糖苷的水平进行了观察。在整个种质组中，r（2）> Q（95个非连锁位点对）显著LD的连锁SSR位点对比例为6.29%。此外，LD随距离迅速衰减，这将允许使用我们的种质集在全基因组关联研究中具有相对较高的定位分辨率，但另一方面，将需要大量的标记。
Rapeseed (Brassica napus L.) is the leading European oilseed crop serving as source for edible oil and renewable energy. The objectives of our study were to (i) examine the population structure of a large and diverse set of B. napus inbred lines, (ii) investigate patterns of genetic diversity within and among different germplasm types, (iii) compare the two genomes of B. napus with regard to genetic diversity, and (iv) assess the extent of linkage disequilibrium (LD) between simple sequence repeat (SSR) markers. Our study was based on 509 B. napus inbred lines genotyped with 89 genome-specific SSR primer combinations. Both a principal coordinate analysis and software STRUCTURE revealed that winter types, spring types, and swedes were assigned to three major clusters. The genetic diversity of winter oilseed rape was lower than the diversity found in other germplasm types. Within winter oilseed rape types, a decay of genetic diversity with more recent release dates and reduced levels of erucic acid and glucosinolates was observed. The percentage of linked SSR loci pairs in significant (r (2) > Q (95 unlinked loci pairs)) LD was 6.29% for the entire germplasm set. Furthermore, LD decayed rapidly with distance, which will allow a relatively high mapping resolution in genome-wide association studies using our germplasm set, but, on the other hand, will require a high number of markers.