Vaginal Lactobacillus iners abundance is associated with outcome in antibiotic treatment of bacterial vaginosis and capable of inhibiting Gardnerella.
Vaginal Lactobacillus iners abundance is associated with outcome in antibiotic treatment of bacterial vaginosis and capable of inhibiting Gardnerella.
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阴道乳酸杆菌的丰度与细菌性阴道病的抗生素治疗相关，并能够抑制Gardnerella。
DOI：
10.3389/fcimb.2022.1033431
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发表时间：
2022
影响因子：
5.7
通讯作者：
中科院分区：
文献类型：
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作者：
细菌性阴道炎的特征是微生物群落失调，乳酸杆菌属减少，多种厌氧菌增殖，其中加德纳菌属、普雷沃菌属和阿托波菌属位列丰度最高的前三类。目前已鉴定出9种加德纳菌基因组种，但它们的分布情况或任何特定乳酸杆菌种类与细菌性阴道炎的发生或预后之间的关联仍存在争议。本研究共招募了308名患者和62名前来进行年度检查的健康女性，最终纳入了130名符合纳入标准的细菌性阴道炎患者和41名健康女性。采集阴道样本进行显微镜检查、16S rRNA测序、细菌培养与分离。选用阴道加德纳菌、阴道芬内希菌（曾称阴道阿托波菌）和惰性乳酸杆菌的分离菌株进行竞争试验。我们发现，与健康人相比，细菌性阴道炎患者中加德纳菌属、普雷沃菌属和阿托波菌属的相对丰度升高（p<0.0001），但不同临床结局的患者之间未发现显著差异（p>0.05）。9种加德纳菌基因组种中有7种在细菌性阴道炎患者和健康女性中均有检出，且所有检测到的基因组种在细菌性阴道炎患者中的相对丰度更高（p<0.05）。治愈患者的GS03水平高于病情处于中间状态和治疗失败的患者（p = 0.005，0.0337）。治愈患者体内的惰性乳酸杆菌显著多于其他两组（p = 0.0021，p<0.0001），且其抑制阴道加德纳菌和阴道芬内希菌生长的能力得到了验证。总之，在中国细菌性阴道炎患者中检测到7种加德纳菌基因组种，但未发现其分布与细菌性阴道炎的发生或预后存在关联。惰性乳酸杆菌在治愈患者中的相对丰度更高，且通过体外抑制实验验证了其对阴道加德纳菌和阴道芬内希菌的抗菌活性。惰性乳酸杆菌可能成为细菌性阴道炎患者临床结局的预测指标，其抗菌功能可能对细菌性阴道炎患者有益。
Bacterial vaginosis is characterized as a polymicrobial dysbiosis with the loss of Lactobacillus spp. and growth of multiple anerobic bacteria, including Gardnerella, Prevotella and Atopobium ranked as the top three most abundant. A total of nine Gardnerella genomospecies have been identified, yet the association between their distribution or any exact Lactobacillus species with BV occurrence or prognosis remains controversial. A total of 308 patients and 62 healthy women who sought annual examinations were recruited, with 130 BV patients and 41 healthy women who met our inclusion criteria finally included. Vaginal samples were used for microscopic examination, 16S rRNA sequencing, bacterial culture and isolation. Isolates of Gardnerella vaginalis, Fannyhessae vaginae (used to be called Atopobium vaginae) and Lactobacillus iners were used for competition tests. We found that the relative abundances of Gardnerella, Prevotella and Atopobium were elevated in BV patients compared to healthy people (p<0.0001), yet no significant differences were found among patients with different clinical outcomes (p>0.05). Seven out of nine Gardnerella genomospecies were present in both BV patients and healthy women, and the relative abundances of all detected genomospecies were higher in BV patients (p<0.05). Cured patients possessed higher GS03 than intermediate and failed patients (p=0.005, 0.0337). L. iners was significantly higher in cured patients than in the other two groups (p=0.0021, p<0.0001), and its ability to inhibit the growth of G. vaginalis and F. vaginae was validated. In summary, seven Gardnerella genomospecies were detected in Chinese BV patients, but no association of its distribution and BV occurrence or prognosis was found. The relative abundance of L. iners was higher in cured patients, and its antimicrobial activity against G. vaginalis and F. vaginae was validated through in vitro inhibition experiment. L. iners could become a predictive indicator of clinical outcomes of BV patients, and its antimicrobial function might be beneficial to BV patients.