ENHANCEMENT OF SENSITIVITY OF HUMAN SQUAMOUS CARCINOMA-CELLS TO RADIATION BY EPIDERMAL GROWTH-FACTOR
ENHANCEMENT OF SENSITIVITY OF HUMAN SQUAMOUS CARCINOMA-CELLS TO RADIATION BY EPIDERMAL GROWTH-FACTOR
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DOI：
10.1093/jnci/81.13.1020
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发表时间：
1989-07-05
影响因子：
10.3
通讯作者：
SUTHERLAND, RM
中科院分区：
文献类型：
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作者：
KWOK, TT;SUTHERLAND, RM
进行实验以确定鼠表皮生长因子 (EGF) 对以指数单层培养物生长的人鳞状癌细胞系 CaSki 的辐射反应的影响。研究的辐射反应包括从潜在致命损伤中恢复（PLDR）和从亚致死损伤中恢复（SLDR）。辐射后（在克隆形成测定期间）或辐射之前、期间和之后连续存在的细胞培养物中 EGF 的存在增强了细胞的放射敏感性并降低了其铺板效率 (PE)。然而，当 EGF 仅在照射前和照射期间存在于细胞培养物中时，这些效果就不那么大了。放射敏感性的增强与细胞存活曲线肩部区域的减少相关。当 EGF/mL 为 10 mg 时，PE 降低和放射敏感性增强达到最大。然而，EGF 持续存在于细胞培养物中，包括在 6 小时修复期间，对细胞 PLDR 或 SLDR 没有影响。照射后细胞中出现大量单链DNA断裂，6小时后恢复到对照水平。此外，照射前 48 小时和 6 小时修复期间细胞培养物中存在的 EGF 对对照细胞或照射细胞的 DNA 碱性洗脱曲线没有影响。 EGF不影响未照射细胞的生长；然而，它延长了受辐射细胞生长的滞后期。总之，这种 EGF 诱导的放射敏感性增强与 EGF 对 PE、细胞生长、PLDR 或 SLDR 的影响不相关。
Experiments were done to determine the effect of murine epidermal growth factor (EGF) on the radiation response of the human squamous carcinoma cell line CaSki grown as an exponential monolayer culture. The radiation responses studied included recovery from potentially lethal damage (PLDR) and recovery from sublethal damage (SLDR). The presence of EGF in the cell culture either after irradiation (during the clonogenic assay period) or continuously before, during, and after irradiation enhanced the radiosensitivity of the cells and reduced their plating efficiency (PE). However, these effects were not as great when EGF was present in the cell culture only before and during irrdiation. This enhancement of radiosensitivity was associated with a reduction in the shoulder region of the cell survival curves. The PE reduction and radiosensitivity enhancement were maximum with 10 mg of EGF/mL. However, EGF present continuously in the cell culture, including during the 6-hour repair period, had no effect on cellular PLDR or SLDR. The single-stranded DNA breaks present in large numbers in cells immediately after irradiation returned to control levels by 6 hours. Moreover, EGF present in cell cultures for the 48 hours before irradiation and during the 6-hour repair period had no effect on the DNA alkaline elution profiles of either control or irradiated cells. EGF did not affect the growth of unirradiated cells; however, it extended the lag-phase period for growth of irradiated cells. In summary, this EGF-induced radiosensitivity enhancement was not correlated with the effects of EGF on PE, cell growth, PLDR, or SLDR.