Changes in the fluorescence of the Caribbean coral Montastraea faveolata during heat‐induced bleaching
Changes in the fluorescence of the Caribbean coral Montastraea faveolata during heat‐induced bleaching
复制标题
热致白化过程中加勒比珊瑚 Montastraea faveolata 荧光的变化
DOI：
--
复制
发表时间：
2003
期刊：
影响因子：
--
通讯作者：
J. Jaffe
中科院分区：
文献类型：
--
作者：
David G. Zawada1;J. Jaffe
为了评估常见的绿色和橙色荧光主体颜料的响应，对加勒比珊瑚 Montastraea faveolata 样本进行了热应力实验。从巴哈马李斯托金岛附近的一个小海洋珊瑚礁采集了七对样本。 14 颗珊瑚中的 7 颗经历了 28 天的高温，随后经历了持续 53 天的恢复期。在整个实验过程中，在与绿色和橙色主体色素以及叶绿素相对应的波长下记录了诱导荧光的高分辨率（~400 µm Pixel−1）多光谱图像。这些图像显示，两种主体色素的荧光集中在息肉中心，并在息肉之间的区域下降 70-90%。然而，叶绿素荧光几乎均匀地分布在整个珊瑚表面，但在息肉中心周围减少了 10-30%。开发了绿色和橙色颜料之间的归一化差异比（GO 比），以便于与作为漂白指示剂的叶绿素荧光进行比较。分析显示，GO 比率持续低于零与珊瑚死亡之间存在高度相关性。最后，这个比例可以抵抗其他叶绿素荧光来源的污染，例如丝状荧光
In order to evaluate the response of commonly occurring green and orange fluorescent host‐based pigments, a thermal stress experiment was performed on specimens of the Caribbean coral Montastraea faveolata. Seven paired samples were collected from a small oceanic reef near Lee Stocking Island in the Bahamas. Seven of the fourteen corals were subjected to elevated temperatures for 28 d, followed by a recovery period lasting 53 d. Throughout the experiment, high‐resolution (~400 µm pixel−1) multispectral images of induced fluorescence were recorded at wavelengths corresponding to the green and orange host pigments, plus chlorophyll. These images revealed that the fluorescence of both host pigments was concentrated at polyp centers and declined by 70–90% in regions between polyps. Chlorophyll fluorescence, however, was distributed almost uniformly across the entire coral surface, but with decreases of 10–30% around polyp centers. A normalized difference ratio between the green and orange pigments (GO ratio) was developed to facilitate comparison with chlorophyll fluorescence as a bleaching indicator. Analysis showed a high correspondence between a sustained GO ratio of less than zero and the death of corals. Finally, this ratio was resistant to contamination from other sources of chlorophyll fluorescence, such as filamentous