Detection and analyses of ascorbyl radical in cerebrospinal fluid and serum of acute lymphoblastic leukemia
Detection and analyses of ascorbyl radical in cerebrospinal fluid and serum of acute lymphoblastic leukemia
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DOI：
10.1006/abio.1997.2372
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发表时间：
1997-12-01
影响因子：
2.9
通讯作者：
Miura, Y
中科院分区：
文献类型：
--
作者：
Nakagawa, K;Kanno, H;Miura, Y
本文首次在室温下用电子顺磁共振（EPR）技术对急性淋巴细胞白血病（ALL）患者脑脊液（CSF）中的自由基进行了检测和分析，并介绍了另一种测量自由基的毛细管方法，自由基的EPR谱显示出特征性的双峰，其超精细耦合值为1.8G;并且g = 2.005，基于EPR测量、计算机模拟和文献值，我们已经确定该物种是抗坏血酸自由基（AsR）。已经在来自未治疗、正在接受化疗和治疗后的ALL患者的CSF样品中研究了该自由基，用已知浓度的硝酰基自由基测定脑脊液和血清中的抗坏血酸自由基浓度。另外，对急性淋巴细胞白血病患者的脑脊液和血清进行了沿着比较，并对所得数据进行统计学分析。结果表明：化疗患者脑脊液和血清中AsR有较强的相关性（n = 57，r = 0.57，P < 0.0001），抗坏血酸在治疗中与血清中有较好的相关性，但在治疗后与脑脊液中抗坏血酸无相关性。(C)中国科学出版社.
We have detected and analyzed a free radical in human cerebrospinal fluid (CSF) of acute lymphoblastic leukemia (ALL) for the first time using electron paramagnetic resonance (EPR) at ambient temperature, We have also introduced an alternative capillary method to measure the radical, EPR spectra of the rad ical show a characteristic doublet with hyperfine coupling value of 1.8 G; and g = 2.005, Based on EPR measurements, computer simulation, and literature values, we have determined that the species is ascorbyl radical (AsR), The radical has been investigated in CSF samples from ALL patients having no therapy, undergoing chemotherapy, and following therapy, Determination of the ascorbyl radical concentrations in CSF and serum was attempted using known concentrations of a nitroxyl radical. In addition, comparison in CSF and serum for ALL has been made along with statistical analyses of the data obtained, We found that AsR in CSF and serum has a strong correlation in patients undergoing chemotherapy (n = 57, r = 0.57, P < 0.0001), Ascorbate in CSF and serum show good correlation in patients having therapy but not for patients after therapy. (C) Academic Press.