Fiber-optic fluorescence correlation spectrometer.

Fiber-optic fluorescence correlation spectrometer.
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光纤荧光相关光谱仪。

DOI:
10.1364/ao.45.007538
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发表时间:
2006
期刊:
影响因子:
1.9
通讯作者:
S. Maiti
S. Maiti
中科院分区:
工程技术4区
文献类型:
--
作者:
K. Garai;M. Muralidhar;S. Maiti

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荧光相关光谱(FCS)是一种用于检测稀溶液中探针的大小、浓度、流速和反应动力学的灵敏技术。传统的FCS光谱仪以使用笨重的光学元件为代价来实现这种灵敏度。我们展示了一种利用3.3微米模场直径的单模光纤进行FCS测量的技术。我们证明,该技术具有足够的灵敏度来对半径为13 nm的荧光珠进行FCS测量,并且结果与理论预测一致。我们的方法潜在地允许FCS扩展到远程和活体应用。
Fluorescence correlation spectroscopy (FCS) is a sensitive technique used to probe size, concentration, flow velocity, and reaction kinetics in a dilute solution. Conventional FCS spectrometers achieve this sensitivity at the cost of using bulky optics. We demonstrate a technique that utilizes a single-mode optical fiber of 3.3 microm mode field diameter to perform FCS measurements. We demonstrate that the technique has adequate sensitivity to perform FCS measurements on fluorescent beads of 13 nm radius, and that the results agree with theoretical predictions. Our method potentially allows FCS to be extended to remote and in vivo applications.
DOI: 10.1227/00006123-199512000-00017
发表时间: 1995-12
期刊: Neurosurgery
影响因子: 4.8
作者:
A. Zauner;R. Bullock;X. Di;H. Young
通讯作者: A. Zauner;R. Bullock;X. Di;H. Young