Self-Assembly of Multi-nanozymes to Mimic an Intracellular Antioxidant Defense System
Self-Assembly of Multi-nanozymes to Mimic an Intracellular Antioxidant Defense System
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多种纳米酶的自组装模拟细胞内抗氧化防御系统
DOI：
10.1002/anie.201600868
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发表时间：
2016-06-01
影响因子：
16.6
通讯作者：
Qu, Xiaogang
中科院分区：
文献类型：
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作者：
Huang, Yanyan;Liu, Zhen;Qu, Xiaogang
本研究首次构建了一种新型的多纳米酶协同平台来模拟细胞内抗氧化酶防御系统。V2O5纳米线模拟谷胱甘肽过氧化物酶（GPx），MnO2纳米颗粒模拟超氧化物歧化酶（SOD）和过氧化氢酶（CAT）。多巴胺被用作连接剂来实现纳米材料的组装。所获得的V2O5@pDA@MnO2纳米复合物可以作为一种多纳米酶模型来模拟细胞内基于抗氧化酶的防御过程，其中例如SOD、CAT和GPx共同参与。此外，通过与多巴胺的组装，杂化纳米复合材料提供了协同抗氧化作用。重要的是，体外和体内实验都表明，我们的生物相容性系统表现出优异的细胞内活性氧（ROS）清除能力，以保护细胞成分免受氧化应激，显示其在炎症治疗中的潜在应用。
In this work, for the first time, we constructed a novel multi-nanozymes cooperative platform to mimic intracellular antioxidant enzyme-based defense system. V2O5 nanowire served as a glutathione peroxidase (GPx) mimic while MnO2 nanoparticle was used to mimic superoxide dismutase (SOD) and catalase (CAT). Dopamine was used as a linker to achieve the assembling of the nanomaterials. The obtained V2O5@pDA@MnO2 nanocomposite could serve as one multi-nanozyme model to mimic intracellular antioxidant enzyme-based defense procedure in which, for example SOD, CAT, and GPx co-participate. In addition, through assembling with dopamine, the hybrid nanocomposites provided synergistic antioxidative effect. Importantly, both in vitro and in vivo experiments demonstrated that our biocompatible system exhibited excellent intracellular reactive oxygen species (ROS) removal ability to protect cell components against oxidative stress, showing its potential application in inflammation therapy.