Stage specific, (O4+GalC-) isolated oligodendrocyte progenitors produce MBP+ myelin in vivo.
Stage specific, (O4+GalC-) isolated oligodendrocyte progenitors produce MBP+ myelin in vivo.
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DOI：
10.1159/000111652
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发表时间：
1992
影响因子：
2.9
通讯作者：
A. Warrington;Elisa Barbarese;S. E. Pfeiffer
中科院分区：
文献类型：
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作者：
A. Warrington;Elisa Barbarese;S. E. Pfeiffer
O4+/A007+GalC- 前少突胶质细胞代表少突胶质细胞谱系的一个独特的发育阶段，发生在有丝分裂后 GalC+ 少突胶质细胞出现之前。这些细胞通过免疫淘选程序直接从出生后的大鼠端脑中分离出来，当移植回体内环境时，可以最终分化并形成髓鞘轴突。具体来说，30天后，在新生颤抖小鼠宿主的大脑中，O4+GalC-少突胶质祖细胞产生了髓磷脂碱性蛋白阳性（MBP+）斑块。通过光学和共聚焦显微镜检查，这些 MBP+ 斑块含有少突胶质细胞体和鞘状宿主颤抖者轴突，其形态与类似年龄的正常大鼠大脑中发现的轴突相似。这些结果表明，分离的O4+GalC-细胞可以成为生化成熟的少突胶质细胞，具有形成髓鞘的能力，并进一步确定了少突胶质细胞在接受环境下保留其髓鞘化轴突能力的发育时期。
O4+/A007+GalC- proligodendroblasts represent a distinct stage of development in the oligodendrocyte lineage, occurring just prior to the appearance of postmitotic GalC+ oligodendrocytes. These cells, isolated directly from postnatal rat telencephalon by an immunopanning procedure, can terminally differentiate and myelinate axons when transplanted back into an in vivo environment. Specifically, after 30 days in the brain of newborn shiverer mouse hosts, O4+GalC- oligodendrocyte progenitors produced myelin basic protein positive (MBP+) patches. These MBP+ patches, examined by both light and confocal microscopy, contained oligodendrocyte cell bodies and ensheathed host shiverer axons morphologically similar to those found in normal rat brain at an analogous age. These results suggest that isolated O4+GalC- cells can become biochemically mature oligodendrocytes with the capacity to elaborate myelin sheaths, and further define the period of development during which oligodendrocytes retain their capacity to myelinate axons when given a receptive environment.