Chitosan-hyaluronic acid hydrogel coated poly(caprolactone) multiscale bilayer scaffold for ligament regeneration
Chitosan-hyaluronic acid hydrogel coated poly(caprolactone) multiscale bilayer scaffold for ligament regeneration
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DOI：
10.1016/j.cej.2014.08.106
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发表时间：
2015-01-15
影响因子：
15.1
通讯作者：
Jayakumar, R.
中科院分区：
文献类型：
--
作者：
Deepthi, S.;Jeevitha, K.;Jayakumar, R.
韧带撕裂，见于年轻运动员和老年人，是一个非常具有挑战性的问题，以实现完全愈合，因为它的细胞减少，减慢了韧带受伤后的再生。组织工程是一种韧带再生的方法，它利用模拟自然环境的工程化支架来优化细胞生物材料相互作用的响应，以加快再生速度。静电纺丝技术和水凝胶对设计韧带的结构很有用。本研究制备了PCL取向微纤维/随机纳米纤维(PCL取向多尺度纤维)和PCL随机微纤维/纳米纤维(PCL随机多尺度纤维)电纺多尺度纤维支架。将壳聚糖透明质酸水凝胶涂层在这些纤维支架上，并将其分层，形成双层结构。用扫描电子显微镜、傅里叶变换红外光谱和拉伸测试对所制备的支架进行了表征。蛋白质吸附研究表明，与未涂层支架相比，涂层支架对蛋白质的吸附效果更好，这进一步提高了细胞活力，阿拉玛蓝法测定细胞存活率，Pico绿法测定DNA含量。兔韧带成纤维细胞在支架上的附着和渗透研究表明，细胞沿排列的纤维延长，这将有利于在自然韧带环境中沿力方向排列细胞。水凝胶涂层在PCL随机多尺度纤维上表现出较好的细胞渗透性。这项研究表明，使用水凝胶涂层系统来提供细胞和营养物质的储存库，这些系统的进一步改进将使其有望用于韧带再生。(C)2014爱思唯尔B.V.保留所有权利。
Ligament tears, seen in young athletes to elderly people, pose a very challenging problem to achieve complete healing, due to its hypocellularity that decelerate regeneration of ligament after injury. Tissue engineering is an approach for the ligament regeneration that optimizes the response of cell biomaterial interaction to fasten regeneration using engineered scaffolds that mimic the native environment. Electrospinning techniques and hydrogels are useful to engineer the structure of ligament. In this study, electrospun multiscale fibrous scaffold of PCL aligned microfibers/random nanofibers (PCL aligned multiscale fibers) and PCL random microfibers/nanofibers (PCL random multiscale fibers) were developed. Chitosan hyaluronic acid hydrogel coating was done on these fibrous scaffolds and this was layered to form a bilayered construct. The developed scaffold was characterized by SEM, FTIR, and tensile testing. Protein adsorption studies show better protein adsorption on coated scaffolds compared to uncoated scaffolds which further improved the cell viability as determined by Alamar blue assay and DNA quantification by Pico green assay. Rabbit ligament fibroblast cell attachment and infiltration study conducted on the scaffolds showed cell elongation along the aligned fibers, which would be advantageous in the need to align cells along the direction of force in native ligament environment. Hydrogel coating on PCL random multiscale fibers show better cell infiltration. This study implies the use of hydrogel coated systems to provide a reservoir for cells and nutrients and further modifications of these systems would make it promising for ligament regeneration. (C) 2014 Elsevier B.V. All rights reserved.