VARIABLE COMPATIBILITY OF CLONED ALNUS-GLUTINOSA ECOTYPES AGAINST INEFFECTIVE FRANKIA STRAINS
VARIABLE COMPATIBILITY OF CLONED ALNUS-GLUTINOSA ECOTYPES AGAINST INEFFECTIVE FRANKIA STRAINS
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DOI：
10.1007/bf02370552
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发表时间：
1988-01-01
期刊：
影响因子：
4.9
通讯作者：
AKKERMANS, ADL
中科院分区：
文献类型：
--
作者：
HAHN, D;STARRENBURG, MJC;AKKERMANS, ADL
对桤木生态型（森林生态型、先锋生态型）的体外繁殖克隆与源自两种生态型的 Frankia 菌株进行的结瘤试验表明，寄主植物相容性存在差异。先锋生态型克隆的接种植物没有被无法在纯培养物中固氮的菌株感染。只能在森林生态型的克隆上诱导结瘤，但不能检测到固氮活性。通过 SEM 和 TEM 对结节的超微结构观察表明，这些菌株的无效性与感染细胞中缺乏囊泡有关。细胞仅充满菌丝：孢子囊和囊泡均检测不到。相反，在接种有效菌株后，两个桤木克隆都可以获得有效的结节，表明感染细胞中囊泡簇的正常发育。在纯培养中，无效菌株不产生囊泡；孢子囊仅在生长早期发现。结果表明存在对桤木不同克隆无感染性或无效的Frankia菌株。
Nodulation tests on in-vitro propagated clones of Alnus glutinosa ecotypes (forest ecotype, pioneer ecotype) with Frankia strains originating from both ecotypes indicated differences in host-plant compatibility. Inoculated plants of the pioneer ecotype clone were not infected by strains, that were unable to fix nitrogen in pure culture. Nodulation could only be induced on the clone of the forest ecotype, but no nitrogen-fixing activity could be detected. Ultra-structural observations of the nodules by SEM and TEM indicated that ineffectivity of these strains was correlated with the lack of vesicles in the infected cells. Cells were only filled with hyphae: neither sporangia nor vesicles could be detected. In contrast, effective nodules could be obtained on both alder clones after inoculation with an effective strain, showing normal development of vesicle clusters in infected cells. In pure culture the ineffective strains produced no vesicles; sporangia were found only during early stage of growth. The results demonstrate the existence of Frankia strains which were either non-infective or ineffective on different clones of Alnus glutinosa.