XYLANASE PRODUCTION IN ASPERGILLUS-NIDULANS - INDUCTION AND CARBON CATABOLITE REPRESSION

XYLANASE PRODUCTION IN ASPERGILLUS-NIDULANS - INDUCTION AND CARBON CATABOLITE REPRESSION
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DOI:
10.1016/0378-1097(94)90033-7
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发表时间:
1994-01-15
影响因子:
2.1
通讯作者:
RAMON, D
RAMON, D
中科院分区:
生物学4区
文献类型:
--
作者:
PINAGA, F;FERNANDEZESPINAR, MT;RAMON, D

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研究了不同来源的木聚糖、单糖、低聚木糖和木糖衍生物等多种化合物对真菌nidulans胞外木聚糖酶合成的诱导作用。某些木聚糖(小麦阿拉伯木聚糖、燕麦木聚糖、桦木木聚糖和4-O-甲基-D-葡萄糖醛-D-木聚糖)被发现是最有效的诱导剂。此外,低聚木糖如木二糖、木三糖和木四糖也是诱导剂,它们的效率与它们的链长直接相关。相反,木糖并不是一个真正的诱导剂。该菌分泌的三种内切β-(1,4)-木聚糖酶中,24 kDa的酶不受碳分解产物的抑制,而另外两种酶(22 kDa和34 kDa)则受CREA基因产物介导的葡萄糖抑制。
The induction of the synthesis of extracellular xylanases was investigated in the fungus Aspergillus nidulans using a number of compounds, including xylans of different origin, monosaccharides, xylooligosaccharides and xylose derivatives. Certain xylans (wheat arabinoxylan, oat spelt xylan, birchwood xylan and 4-O-methyl-D-glucurono-D-xylan) were found to be the most powerful inducers. Also, xylooligosaccharides such as xylobiose, xylotriose and xylotetraose served as inducers, their efficiency being directly related to their chain length. Xylose, on the contrary, was not a true inducer. Of the three endo-beta-(1,4)-xylanases secreted by A. nidulans, that of 24 kDa was not under carbon catabolite repression, whereas the other two, of 22 and 34 kDa, were under glucose repression mediated by the creA gene product.