Mammary epithelial cells support and transfer productive human T-cell lymphotropic virus infections.
Mammary epithelial cells support and transfer productive human T-cell lymphotropic virus infections.
复制标题
乳腺上皮细胞支持并转移高效的人类 T 细胞嗜淋巴细胞病毒感染。
DOI：
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发表时间：
1998
期刊：
影响因子：
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通讯作者：
P. Southern
中科院分区：
文献类型：
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作者：
R. J. LeVasseur;Š. Southern;P. Southern
目标
研究管腔和基底人类乳腺上皮细胞（HMEC）是否易受人类T细胞嗜淋巴细胞病毒I型和II型（HTLV-I和HTLV-II）的生产性感染，以及乳腺上皮细胞的HTLV感染是否有助于接种具有HTLV感染性的乳汁，并支持病毒从母亲传播给哺乳期婴儿。
研究设计/方法
通过与丝裂霉素C处理的HTLV生产T细胞系和HTLV感染的乳上皮细胞共培养，感染基底上皮细胞的原代培养物，并通过聚合酶链反应（PCR）扩增和免疫细胞化学染色监测感染的转移。
结果
发现乳腺基底上皮细胞对HTLV感染敏感，并且能够将HTLV感染转移到正常外周血淋巴细胞（PBL）。
结论
乳腺上皮细胞中可能存在HTLV感染性的储存库，并通过感染穿过上皮的淋巴细胞以及通过将感染的上皮细胞、感染性细胞片段和游离病毒体直接释放到乳汁中，有助于将HTLV感染性引入乳汁中。
OBJECTIVES
To investigate whether luminal and basal human mammary epithelial cells (HMEC) are susceptible to productive infection by human T-cell lymphotropic virus types I and II (HTLV-I and HTLV-II) and whether HTLV infection of breast epithelial cells could contribute to the seeding of milk with HTLV infectivity and support virus transmission from mother to nursing infant.
STUDY DESIGN/METHODS
Primary cultures of basal epithelial cells were infected by coculture with mitomycin-C-treated HTLV-producer T-cell lines and HTLV-infected milk epithelial cells, and the transfer of infection was monitored by polymerase chain reaction (PCR) amplification and immunocytochemical staining.
RESULTS
Basal mammary epithelial cells were found to be susceptible to HTLV infection and capable of transferring HTLV infection to normal peripheral blood lymphocytes (PBL).
CONCLUSIONS
A reservoir for HTLV infectivity could exist in mammary epithelial cells and contribute to the introduction of HTLV infectivity into milk by infecting lymphocytes that traverse the epithelium and by the release of infected epithelial cells, infectious cell fragments, and free virions directly into the milk.