TP53 mutation analyses on breast carcinomas: a study of paraffin-embedded archival material.
TP53 mutation analyses on breast carcinomas: a study of paraffin-embedded archival material.
复制标题
TP53对乳腺癌的突变分析：石蜡包裹的档案材料的研究。
DOI：
10.1038/bjc.1996.400
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发表时间：
1996-08
影响因子：
8.8
通讯作者：
Eyfjord, J E
中科院分区：
文献类型：
--
作者：
Gretarsdottir, S;Tryggvadottir, L;Jonasson, J G;Sigurdsson, H;Olafsdottir, K;Agnarsson, B A;Ogmundsdottir, H;Eyfjord, J E
The aim of this investigation was to examine the possibility of analysing TP53 mutations in archival paraffin-embedded material with the constant denaturant gel electrophoresis (CDGE) method. We extracted DNA from 193 archival primary breast carcinoma samples, diagnosed in 1981-83; further analysis was possible for 186 of these. TP53 mutations in exons 5-8 were detected with CDGE in 30 samples (16.1%) and 17 of these mutations were confirmed by sequencing. Immunohistochemistry demonstrated TP53 nuclear accumulation in 58 tumours (31%). A strong association between the presence of TP53 mutations and TP53 immunostaining was observed (P < 0.001). Our mutation and immunohistochemistry results are in agreement with other findings based on fresh tumour tissue. TP53 abnormalities were significantly related to high S-phase fraction, low oestrogen receptor (ER) content and high tumour grade. Survival of patients with TP53 abnormalities, in the group as a whole, did not differ from patients with normal TP53. Our study did, however, show that patients with abnormal TP53 had a significantly shorter post-recurrence survival (P = 0.005) than patients with normal TP53.
