Vaccination with Bivalent DNA Vaccine of α1-Giardin and CWP2 Delivered by Attenuated Salmonella typhimurium Reduces Trophozoites and Cysts in the Feces of Mice Infected with Giardia lamblia.
Vaccination with Bivalent DNA Vaccine of α1-Giardin and CWP2 Delivered by Attenuated Salmonella typhimurium Reduces Trophozoites and Cysts in the Feces of Mice Infected with Giardia lamblia.
复制标题
接种由减毒鼠伤寒沙门氏菌提供的 α1-Giardin 和 CWP2 双价 DNA 疫苗可减少感染蓝氏贾第鞭毛虫的小鼠粪便中的滋养体和囊肿
DOI：
10.1371/journal.pone.0157872
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Wang HY
中科院分区：
文献类型：
--
作者：
Feng XM;Zheng WY;Zhang HM;Shi WY;Li Y;Cui BJ;Wang HY
蓝氏贾第鞭毛虫是人类最常见的传染性原虫之一，可引起旅行者腹泻。寻找能有效诱导保护性免疫的抗原已成为贾第虫病疫苗研制的关键。方法学/主要发现用G.用减毒鼠伤寒沙门氏菌SL 7027经口给药的λ氏虫滋养体特异性α1-贾第丁DNA疫苗，与PBS缓冲液对照相比，滋养体减少74.2%，但包囊脱落仅减少28%。用沙门氏菌递送的囊特异性CWP 2 DNA口服疫苗接种，使接种疫苗的小鼠粪便中的囊脱落减少89%。值得注意的是，用沙门氏菌递送的二价α1-贾第丁和CWP 2 DNA疫苗接种的小鼠在接种小鼠的粪便中产生滋养体（79%）和包囊（93%）的显著减少。这种寄生虫减少与免疫小鼠中强烈的局部粘膜伊加分泌和IgG 2a-显性全身免疫应答相关。结论以α1-贾第虫素和CWP 2为靶点的双价疫苗能保护小鼠免受贾第虫滋养体的定植，同时阻断宿主包囊的转化，可用于预防贾第虫感染和阻断贾第虫病的传播。
Background Giardia lamblia is one of the most common infectious protozoans in human that may cause diarrhea in travelers. Searching for antigens that induced effectively protective immunity has become a key point in the development of vaccine against giardiasis. Methodology/Principal Findings Mice vaccinated with G. lamblia trophozozite-specific α1-giardin DNA vaccine delivered orally by attenuated Salmonella typhimurium SL7027 elicited 74.2% trophozoite reduction, but only 28% reduction in cyst shedding compared with PBS buffer control. Oral vaccination with Salmonella-delivered cyst-specific CWP2 DNA produced 89% reduction in cysts shedding in feces of vaccinated mice. Significantly, the mice vaccinated with Salmonella-delivered bivalent α1-giardin and CWP2 DNA vaccines produced significant reduction in both trophozoite (79%) and cyst (93%) in feces of vaccinated mice. This parasite reduction is associated with the strong local mucosal IgA secretion and the IgG2a-dominant systemic immune responses in vaccinated mice. Conclusions The results demonstrate that bivalent vaccines targeting α1-giardin and CWP2 can protect mice against the colonization of Giardia trophozoite and block the transformation of cyst in host at the same time, and can be used to prevent Giardia infection and block the transmission of giardiasis.