Multianalyte pin-printed biosensor arrays based on protein-doped xerogels
Multianalyte pin-printed biosensor arrays based on protein-doped xerogels
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DOI：
10.1021/ac020454
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发表时间：
2002-12-15
影响因子：
7.4
通讯作者：
Bright, FV
中科院分区：
文献类型：
--
作者：
Cho, EJ;Tao, ZY;Bright, FV
我们报告的第一个生物传感器阵列的基础上针打印蛋白质掺杂干凝胶。各个生物传感器元件的直径大约为100 μ m。阵列形成于（1）由外部源（激光）激发的平面衬底上或（2）直接在发光二极管的表面上。我们说明了我们的方法的潜力，通过制造，测试和表征四种类型的针印刷生物传感器阵列（PPBSA）的同时检测葡萄糖和O-2。PPBSA的分析可靠的操作范围为0.1-10 mM葡萄糖和0.1-100%的O-2。PPBSA表现出短期和长期的可重复性不低于4%和8%，分别。整体阵列间响应再现性小于或等于12%。这些结果首次证明了溶胶-凝胶处理和针式印刷方法的组合作为一种快速形成集成的，可重复使用的，稳定的生物传感器阵列的合奏，同时多分析物检测的方式。
We report the first biosensor arrays based on pin printing protein-doped xerogels. The individual biosensor elements are on the order of 100 mum in diameter. Arrays are formed (1) onto a planar substrate that is excited by an external source (laser) or (2) directly on the face of a light-emitting diode. We illustrate the potential of our approach by fabricating, testing, and characterizing four types of pin-printed biosensor arrays (PPBSA) for the simultaneous detection of glucose and O-2. The analytically reliable operating ranges for the PPBSAs are 0.1-10 mM for glucose and 0.1-100% for O-2. The PPBSAs exhibit short- and long-term reproducibilities of no worse than 4 and 8%, respectively. The overall array-to-array response reproducibilities are less than or equal to 12%. These results demonstrate for the first time the combination sol-gel processing and pin printing methods as a way to rapidly form ensembles of integrated, reusable, and stable biosensor arrays for simultaneous multianalyte detection.