Molecularly Defined Circuitry Reveals Input-Output Segregation in Deep Layers of the Medial Entorhinal Cortex.
Molecularly Defined Circuitry Reveals Input-Output Segregation in Deep Layers of the Medial Entorhinal Cortex.
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DOI：
10.1016/j.neuron.2015.10.041
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发表时间：
2015-12-02
期刊：
影响因子：
16.2
通讯作者：
Nolan MF
中科院分区：
文献类型：
--
作者：
Sürmeli G;Marcu DC;McClure C;Garden DLF;Pastoll H;Nolan MF
内侧内嗅皮层的深层被认为是将信号从海马体传递到其他大脑结构，但是用于将信号路由到深层和从深层路由的通路还没有很好地建立。描绘这些通路对于空间认知和记忆的回路水平理解是重要的。我们发现，在层5a和5b的神经元有不同的分子身份，定义的转录因子Etv 1和Ctip 2，和不同的目标，广泛的端脑内的预测起源于层5a，但不是5b。这种分离的输出是反映了组织的海马神经元的输入从星状细胞在层2和海马，两者优先靶向层5b超过5a。我们的研究结果表明，分子和解剖学组织的输入输出计算在深层的MEC，揭示精确的translaminar微电路，并确定分子定义的路径空间信号影响计算在深层。转录因子Etv 1和Ctip 2区分内嗅层5a和5b层5a具有广泛的端脑内投射，但层5b没有第2层星状细胞的终末，但不是锥体细胞，在深层富集海马和星状细胞的输入优先靶向层5b神经元Sürmeli等。证明了内侧内嗅皮层深层的输入和输出功能在两个细胞群之间划分，这两个细胞群通过其分子身份，椎板位置，translaminar和海马输入以及端脑靶点来区分。
Deep layers of the medial entorhinal cortex are considered to relay signals from the hippocampus to other brain structures, but pathways for routing of signals to and from the deep layers are not well established. Delineating these pathways is important for a circuit level understanding of spatial cognition and memory. We find that neurons in layers 5a and 5b have distinct molecular identities, defined by the transcription factors Etv1 and Ctip2, and divergent targets, with extensive intratelencephalic projections originating in layer 5a, but not 5b. This segregation of outputs is mirrored by the organization of glutamatergic input from stellate cells in layer 2 and from the hippocampus, with both preferentially targeting layer 5b over 5a. Our results suggest a molecular and anatomical organization of input-output computations in deep layers of the MEC, reveal precise translaminar microcircuitry, and identify molecularly defined pathways for spatial signals to influence computation in deep layers. The transcription factors Etv1 and Ctip2 distinguish entorhinal layers 5a and 5b Layer 5a has extensive intratelencephalic projections, but layer 5b does not Terminals of layer 2 stellate, but not pyramidal cells, are enriched in deep layers Hippocampal and stellate cell inputs preferentially target layer 5b neurons Sürmeli et al. demonstrate that input and output functions of the deep layers of the medial entorhinal cortex are divided between two cell populations that are distinguished by their molecular identity, lamina position, translaminar and hippocampal inputs, and telencephalic targets.