Polycomb Proteins Control Floral Determinacy by H3K27me3-mediated Repression of Pluripotency Genes in Arabidopsis thaliana
Polycomb Proteins Control Floral Determinacy by H3K27me3-mediated Repression of Pluripotency Genes in Arabidopsis thaliana
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多梳蛋白通过 H3K27me3 介导的拟南芥多能性基因抑制来控制花决定性
DOI：
10.1093/jxb/erac013
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发表时间：
2022
影响因子：
6.9
通讯作者：
Justin Goodrich
中科院分区：
文献类型：
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作者：
Ralf Müller-Xing;Rhomi Ardiansyah;Qian Xing;Léa Faivre;Jingjing Tian;Guohua Wang;Yucai Zheng;Xue Wang;Tingting Jing;Erica de Leau;Song Chen;Su Chen;Daniel Schubert;Justin Goodrich
Polycomb group(PcG)蛋白介导组蛋白甲基化(H3K27me3)控制着许多发育调节因子在拟南芥中的正确时空表达。干细胞因子WUS在花分生组织(Fms)中的表观遗传沉默取决于PcG蛋白对H3K27me3的沉积。然而，在FM确定过程中，H3K27me3在沉默其他分生组织调节基因和多能性基因中的作用尚未被研究。为此，我们报道了FM停滞期间H3K27me3水平的全基因组动态，以及强烈耗尽的PcG活性对早期花形态发生的影响，包括扩大的和不确定的FM。H3K27me3水平的强烈耗尽导致FM标识基因AGL24的错误表达，这部分导致花逆转导致AP1样花和异位表达WUS和STM的不确定FM。失去STM可以挽救H3K27me3基因缺失的花中多余的花器官和FM的不确定性，这表明FM的过度活动至少部分是由于异位STM表达的结果。尽管如此，WUS仍然是FM活动的关键。我们的结果表明，PcG蛋白在花基因调控网络的多个水平上促进了FM确定性，最初沉默了像AGL24这样的花调控因子，促进了FM的不确定性，随后在FM抑制期间，沉默了分生多能基因，如WUS和STM。
Polycomb group (PcG) protein-mediated histone methylation (H3K27me3) controls the correct spatiotemporal expression of numerous developmental regulators in Arabidopsis. Epigenetic silencing of the stem cell factor WUS in floral meristems (FMs) depends on H3K27me3 deposition by PcG proteins. However, the role of H3K27me3 in silencing of other meristematic regulator and pluripotency genes during FM determinacy has not yet been studied. To this end, we report the genome-wide dynamics of H3K27me3 levels during FM arrest and the consequences of strongly depleted PcG activity on early flower morphogenesis including enlarged and indeterminate FMs. Strong depletion of H3K27me3 levels results in misexpression of the FM identity gene AGL24, which partially leads to floral reversion causing ap1-like flowers and indeterminate FMs expressing ectopically WUS and STM. Loss of STM can rescue supernumerary floral organs and FM indeterminacy in H3K27me3-deficient flowers indicating that the hyperactivity of the FMs is at least partially a result of ectopic STM expression. Nonetheless, WUS remained essential for the FM activity. Our results demonstrate that PcG proteins promote FM determinacy at multi-levels of the floral gene regulatory network, silencing initially floral regulators like AGL24 that promotes FM indeterminacy, and subsequently, meristematic pluripotency genes such as WUS and STM during FM arrest.