Impacts of lesion severity and tyrosine kinase receptor B deficiency on functional outcome of femoral nerve injury assessed by a novel single-frame motion analysis in mice
Impacts of lesion severity and tyrosine kinase receptor B deficiency on functional outcome of femoral nerve injury assessed by a novel single-frame motion analysis in mice
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DOI：
10.1111/j.1460-9568.2005.04274.x
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发表时间：
2005-08-01
影响因子：
3.4
通讯作者：
Schachner, M
中科院分区：
文献类型：
--
作者：
Irintchev, A;Simova, O;Schachner, M
周围神经损伤后的功能恢复往往较差。对于神经科学和医学来说，理解限制或促进功能恢复的细胞和分子机制以及设计有效的治疗方法仍然是严峻的挑战。由于缺乏可靠的方法来评估实验动物的运动功能，进展受到了限制。我们描述了一种新的方法来评估小鼠股神经损伤后的肌肉功能，股神经损伤导致膝关节伸展功能受损。在有或没有体重支持的情况下，通过对动物在运动过程中记录的单个视频帧的角度和距离测量，可以准确地估计功能缺陷。用这种方法，我们描述了股神经损伤和切断后功能恢复的确切时间、过程和程度。此外，我们发现，酪氨酸激酶受体B的表达水平降低的小鼠的功能恢复受到相当大的损害，B受体是神经营养因子脑源性神经营养因子的同源受体。这一发现与脑源性神经营养因子和酪氨酸激酶受体B的已知功能一致，并证明了该方法的潜力。该方法的原理对于在其他外周，特别是中枢神经系统损伤范例中开发新的功能分析是高度相关的。
Functional recovery after peripheral nerve injury is often poor. Comprehension of cellular and molecular mechanisms limiting or promoting restoration of function and design of efficient therapeutic approaches remain serious challenges for neuroscience and medicine. Progress has been restricted by the lack of reliable methods for evaluation of motor functions in laboratory animals. We describe a novel approach for assessment of muscle function in mice after femoral nerve damage, an injury causing impairment of knee extension. The functional deficit can be precisely estimated by angle and distance measurements on single video frames recorded during movements of the animals with or without body weight support. Using this method we describe here the precise time-course and degree of functional recovery after femoral nerve crush and transection. In addition, we show that restoration of function is considerably impaired in mice with a reduced expression level of the tyrosine kinase receptor B, a cognate receptor for the neurotrophin brain-derived neurotrophic factor. This finding is consistent with known functions of brain-derived neurotrophic factor and tyrosine kinase receptor B and demonstrates the potential of the method. The principles of the approach are highly relevant for the development of novel functional assays in other peripheral and, in particular, central nervous system injury paradigms.