Poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering
Poly(lactide-co-glycolide)/hydroxyapatite composite scaffolds for bone tissue engineering
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DOI：
10.1016/j.biomaterials.2005.08.016
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发表时间：
2006-03-01
期刊：
影响因子：
14
通讯作者：
Kim, BS
中科院分区：
文献类型：
--
作者：
Kim, SS;Park, MS;Kim, BS
生物可降解聚合物/生物陶瓷复合骨支架克服了传统陶瓷骨替代材料脆性大、成型困难等缺点。然而，用于制造聚合物/生物陶瓷复合支架的常规方法通常使用有机溶剂（例如，溶剂浇铸和颗粒浸出（SC/PL）方法）。这可能对细胞或组织有害。此外，聚合物溶液可涂覆陶瓷并阻碍它们暴露于支架表面，这可降低接种的成骨细胞与生物活性陶瓷接触的可能性。在这项研究中，一种新的方法，用于制造聚合物/纳米生物陶瓷复合支架与高暴露的生物陶瓷的支架表面的高效骨组织工程开发。在不使用有机溶剂的条件下，采用气体成型颗粒沥滤法（GF/PL）制备了聚（D，L-乳酸-羟基乙酸）/纳米羟基磷灰石（PLGA/HA）复合支架。GF/PL方法在支架表面暴露的HA纳米颗粒显著多于常规SC/PL方法。GF/PL支架显示出互连的多孔结构，没有皮肤层，并表现出上级增强的机械性能的那些由SC/PL方法制造的支架。两种类型的支架与大鼠颅骨成骨细胞接种，并在体外培养或皮下植入到无胸腺小鼠8周。与SC/PL支架相比，GF/PL支架在体外表现出显著更高的细胞生长、碱性磷酸酶活性和矿化。植入后5周和8周再生组织的组织学分析和钙含量定量显示，GF/PL支架上的骨形成比SC/PL支架上更广泛。与SC/PL支架相比，GF/PL支架上增强的骨形成可能是由于HA纳米颗粒在支架表面的更高暴露，其允许与移植细胞直接接触并刺激细胞增殖和成骨分化。这些结果表明，可降解聚合物/生物陶瓷复合支架的新型GF/PL方法制造的骨再生增强与传统的SC/PL方法相比。(c)2005爱思唯尔有限公司保留所有权利。
Biodegradable polymer/bioceramic composite scaffolds call overcome the limitations of conventional ceramic bone substitutes such as brittleness and difficulty in shaping. However, conventional methods for fabricating polymer/bioceramic composite scaffolds often use organic solvents (e.g., the solvent casting and particulate leaching (SC/PL) method),. which might be harmful to cells or tissues. Furthermore, the polymer solutions may coat the ceramics and hinder their exposure to the scaffold surface, which may decrease the likelihood that the seeded osteogenic cells will make contact with the bioactive ceramics. In this study, a novel method for fabricating a polymer/nano-bioceramic composite scaffold with high exposure of the bioceramics to the scaffold surface was developed for efficient bone tissue engineering. poly(D,L-lactic-co-glycolic acid)/nano-hydroxyapatite (PLGA/HA) composite scaffolds were fabricated by the gas forming and particulate leaching (GF/PL) method without the use of organic solvents. The GF/PL method exposed HA nanoparticles at the scaffold surface significantly more than the conventional SC/PL method does. The GF/PL scaffolds showed interconnected porous structures without a skin layer and exhibited superior enhanced mechanical properties to those of scaffolds fabricated by the SC/PL method. Both types of scaffolds were seeded with rat calvarial osteoblasts and cultured in vitro or were subcutaneously implanted into athymic mice for eight weeks. The GF/PL scaffolds exhibited significantly higher cell growth, alkaline phosphatase activity, and mineralization compared to the SC/PL scaffolds in vitro. Histological analyses and calcium content quantification of the regenerated tissues five and eight weeks after implantation showed that bone formation was more extensive oil the GF/PL scaffolds than on the SC/PL scaffolds. Compared to the SC/PL scaffolds, the enhanced bone formation on the GF/PL scaffolds may have resulted from the higher exposure of HA nanoparticles at the scaffold surface, which allowed for direct contact with the transplanted cells and stimulated the cell proliferation and osteogenic differentiation. These results show that the biodegradable polymer/bioceramic composite scaffolds fabricated by the novel GF/PL method enhance bone regeneration compared with those fabricated by the conventional SC/PL method. (c) 2005 Elsevier Ltd. All rights reserved.