Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, Nitrososphaera viennensis.
Nitric Oxide Production from Nitrite Reduction and Hydroxylamine Oxidation by Copper-containing Dissimilatory Nitrite Reductase (NirK) from the Aerobic Ammonia-oxidizing Archaeon, Nitrososphaera viennensis.
复制标题
好氧氨氧化古菌维也纳亚硝化菌中的含铜异化亚硝酸还原酶 (NirK) 通过亚硝酸盐还原和羟胺氧化产生一氧化氮。
DOI：
10.1264/jsme2.me18058
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发表时间：
2018-12-28
影响因子：
2.2
通讯作者：
Oshiki M
中科院分区：
文献类型：
--
作者：
Kobayashi S;Hira D;Yoshida K;Toyofuku M;Shida Y;Ogasawara W;Yamaguchi T;Araki N;Oshiki M
好氧氨氧化古菌（AOA）通过将氨氧化成亚硝酸盐，在全球氮循环中发挥着至关重要的作用，而一氧化氮（NO）是AOA中维持好氧氨氧化活性的关键中间体。我们在此异源表达来自维也纳亚硝化球菌的 NO 形成、含铜、异化亚硝酸还原酶 (NirK)，并研究了其酶学特性。重组蛋白催化 15NO2− 还原为 15NO，羟胺 (15NH2OH) 氧化为 15NO，以及由 15NH2OH 和 14NO2− 生成 14–15N2O。据我们所知，本研究是第一个记录 AOA NirK 酶学特性的研究。
Aerobic ammonia-oxidizing archaea (AOA) play a crucial role in the global nitrogen cycle by oxidizing ammonia to nitrite, and nitric oxide (NO) is a key intermediate in AOA for sustaining aerobic ammonia oxidation activity. We herein heterologously expressed the NO-forming, copper-containing, dissimilatory nitrite reductase (NirK) from Nitrososphaera viennensis and investigated its enzymatic properties. The recombinant protein catalyzed the reduction of 15NO2− to 15NO, the oxidation of hydroxylamine (15NH2OH) to 15NO, and the production of 14–15N2O from 15NH2OH and 14NO2−. To the best of our knowledge, the present study is the first to document the enzymatic properties of AOA NirK.