Cloning of canine Toll-like receptor 9 and its expression in dog tissues
Cloning of canine Toll-like receptor 9 and its expression in dog tissues
复制标题
DOI：
10.1016/j.vetimm.2005.01.010
复制
发表时间：
2005-06-15
影响因子：
1.8
通讯作者：
Hasegawa, A
中科院分区：
文献类型：
--
作者：
Hashimoto, M;Asahina, Y;Hasegawa, A
Toll样受体9(TLR9)被细菌DNA激活，诱导炎性细胞因子的产生。本研究克隆了犬TLR9基因，并进行了序列分析。进一步研究TLR9mRNA在犬组织中的表达。犬TLR9基因全长为3419bp，编码1032个氨基酸。犬TLR9与猫、牛、人、鼠、猪的核苷酸同源性分别为90%、84.3%、83.6%、76.3%和85.2%，氨基酸同源性分别为90.5%、82.7%、81.4%、74.8%和85.7%。逆转录-聚合酶链式反应(RT-PCR)检测到TLR9在犬静脉血单个核细胞和淋巴结中表达，在脾中微弱表达，而在肾、肝、胰腺、肺、小肠、大肠、心脏、皮肤、肌肉、胃和膀胱中未检测到TLR9mRNA。(C)2005 Elsevier B.V.保留所有权利。
Toll-like receptor 9 (TLR9) is activated by bacterial DNA and induces production of inflammatory cytokines. In this study, canine TLR9 cDNA was cloned and sequenced. Further, the expression of TLR9 mRNA was investigated in canine tissues. The full-length cDNA for the canine TLR9 gene was 3419 bp encoding 1032 amino acids. The similarity of canine TLR9 with those of cat, cattle, human, mouse and pig was 90, 84.3, 83.6, 76.3 and 85.2% at the nucleotide level, and 90.5, 82.7, 81.4, 74.8 and 85.7% at the amino acid level. By reverse transcription-polymerase chain reaction (RT-PCR) analysis, canine TLR9 mRNA was detected in venous blood mononuclear cells and lymph node and weakly in spleen, whereas it was not detected in kidney, liver, pancreas, lung, small intestine, large intestine, heart, skin, muscle, stomach and bladder. (c) 2005 Elsevier B.V. All rights reserved.