Reverse transcription of retroviral genomes: mutations in the terminal repeat sequences
Reverse transcription of retroviral genomes: mutations in the terminal repeat sequences
复制标题
逆转录病毒基因组的逆转录：末端重复序列的突变
DOI：
10.1128/jvi.53.2.447-455.1985
复制
发表时间：
1985
影响因子：
5.4
通讯作者：
S. Goff
中科院分区：
文献类型：
--
作者：
L. Lobel;S. Goff
The process of reverse transcription of retroviral genomes begins with the synthesis of a short DNA molecule near the 5' end of the RNA template. This molecule, termed minus-strand strong-stop DNA, is then translocated to the 3' end of the viral RNA by means of a repeated sequence, the R region, present at both ends of the template. The translocation should result in the transfer of genetic information from the 5' R region to the 3' R region. We have generated a series of mutants of Moloney murine leukemia virus with alterations in the R regions by in vitro mutagenesis of a cloned DNA copy of the viral genome. The altered DNAs were introduced into mouse cells by transfection, and the translocation of the mutations during viral replication was assessed. Some mutations were not transferred from the 5' R region to the 3' R region; these results were not in accord with current models for reverse transcription. The results can be explained if DNA molecules shorter than strong-stop DNA, formed by premature termination of synthesis, are sometimes translocated. A number of mutants with large deletions in the R region were tested and were able to replicate with normal strong-stop DNA translocation. Thus, short stretches of homology can be used by the virus to carry out strong-stop translocations.
通过抑制连接子插入诱变构建莫洛尼鼠白血病病毒突变体：可行插入突变的位置。
DOI：
10.1073/pnas.81.13.4149
发表时间：
1984
影响因子：
11.1
作者：
Lobel,LI;Goff,SP
通讯作者：
Goff,SP
哺乳动物细胞中的同源重组介导两个重叠基因片段形成功能基因。
DOI：
10.1073/pnas.80.7.2002
发表时间：
1983
影响因子：
11.1
作者：
deSaintVincent,BR;Wahl,GM
通讯作者：
Wahl,GM