A Comparative Transcriptomic and Proteomic Analysis of Hexaploid Wheat's Responses to Colonization by Bacillus velezensis and Gaeumannomyces graminis, Both Separately and Combined
A Comparative Transcriptomic and Proteomic Analysis of Hexaploid Wheat's Responses to Colonization by Bacillus velezensis and Gaeumannomyces graminis, Both Separately and Combined
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六倍体小麦对贝莱斯芽孢杆菌和全麦酵母单独和组合定植反应的比较转录组学和蛋白质组学分析
DOI：
10.1094/mpmi-03-19-0066-r
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发表时间：
2019-10-01
影响因子：
3.5
通讯作者：
Liu, Changhong
中科院分区：
文献类型：
--
作者：
Kang, Xingxing;Wang, Lanhua;Liu, Changhong
涉及生防剂，病原体和植物的三营养相互作用主要从生防剂的角度进行了分析。为了探索小麦对有益微生物、病原微生物和复合微生物的适应策略，我们首次对小麦根暴露于Bacillus velezensis CC 09、Gaeumannomyces graminis var.分别是三种，以及它们的联合殖民。接种有益B的小麦根的转录或翻译编程。velezensis与致病G.禾谷变种三个月。然而，B. velezensis和G.禾谷变种尽管基因表达模式与G.禾谷变种建议优先防御G.禾谷变种小麦感染。令人惊讶的是，病原相关分子模式触发的免疫和效应触发的免疫使小麦与B预处理。Velezensis对随后的G.禾谷变种感染性三联症另外，B. Velezensis引发了一种依赖于水杨酸（SA）的诱导系统抗性模式，类似于病原体诱导的系统获得性抗性。小麦对坏死型病原菌G.禾谷变种三个月。此外，无论小麦中微生物的类型如何，SA-JA相互作用都导致拮抗效应。进一步增强SA依赖的防御反应，如对联合感染的木质化，
Tritrophic interactions involving a biocontrol agent, a pathogen, and a plant have been analyzed predominantly from the perspective of the biocontrol agent. To explore the adaptive strategies of wheat in response to beneficial, pathogenic, and combined microorganisms, we performed the first comprehensive transcriptomic, proteomic, and biochemical analysis in wheat roots after exposure to Bacillus velezensis CC09, Gaeumannomyces graminis var. tritici, and their combined colonization, respectively. The transcriptional or translational programming of wheat roots inoculated with beneficial B. velezensis showed mild alterations compared with that of pathogenic G. graminis var. tritici. However, the combination of B. velezensis and G. graminis var. tritici activated a larger transcriptional or translational program than for each single microorganism, although the gene expression pattern was similar to that of individual infection by G. graminis var. tritici, suggesting a prioritization of defense against G. graminis var. tritici infection. Surprisingly, pathogen-associated molecular pattern-triggered immunity and effector-triggered immunity made wheat pretreated with B. velezensis more sensitive to subsequent G. graminis var. tritici infection. Additionally, B. velezensis triggered a salicylic acid (SA)-dependent mode of induced systemic resistance that resembles pathogen-induced systemic acquired resistance. Wheat plants mainly depend on SA-mediated resistance, and not that mediated by jasmonic acid (JA), against the necrotrophic pathogen G. graminis var. tritici. Moreover, SA-JA interactions resulted in antagonistic effects regardless of the type of microorganisms in wheat. Further enhancement of SA-dependent defense responses such as lignification to the combined infection was shown to reduce