Suitable light combinations enhance cadmium accumulation in Bidens pilosa L. by regulating the soil microbial communities
Suitable light combinations enhance cadmium accumulation in Bidens pilosa L. by regulating the soil microbial communities
复制标题
DOI：
10.1016/j.envexpbot.2022.105128
复制
发表时间：
2022-11
影响因子：
5.7
通讯作者：
Junting Xie;X. Lou;Yezhen Lu;Hai Huang;Qing Yang;Zhipan Zhang;Wenlu Zhao;Zhiheng Li;Huijun Liu;Shaoting Du;Zhiguo Fang
中科院分区：
文献类型：
--
作者：
Junting Xie;X. Lou;Yezhen Lu;Hai Huang;Qing Yang;Zhipan Zhang;Wenlu Zhao;Zhiheng Li;Huijun Liu;Shaoting Du;Zhiguo Fang
光是影响植物生长的重要自然资源之一，光调控已被认为是加强植物修复的有效手段之一。在这项研究中，单色（红色，蓝色，绿色），双色（红色和蓝色光组合在不同的比例），和三色（红色，蓝色，和绿色光组合在不同的比例）光在相同的强度（100 ± 5 μmol m−2s−1），以提高镉（Cd）积累鬼针草pilosaL。与对照（荧光灯）相比，发光二极管（LED）照射下，植物的转运系数（TF）、生物富集系数（BCF）和总金属提取率（TE）分别提高了13.47- 99.24%、5.33- 39.64%和20.51- 109.62%。在R20 B70 G10（20%红光、70%蓝光和10%绿色光）照射下，植物的BCF和TE值最高，而在R50 B50（50%红光和50%蓝光）和R20 B70 G10照射下，植物的TF值较高。系统聚类和热图分析表明，三色光对植物生长、光合作用、抗氧化酶活性和可溶性蛋白质含量的影响大于双色光和单色光。此外，根际微生物和植物根际促生细菌（如溶菌属和枝杆菌属）的丰度在LED照射下显著增加，沿着土壤酶活性的增强。研究结果表明，R20 B70 G10三色光能有效诱导植物对Cd的提取，并能显著提高三叶鬼针草对Cd污染土壤的修复效果。
Light is one of the natural resources greatly influencing plant growth, and light regulation has been recently considered one of the effective means of enhancing phytoremediation. In this study, monochromatic (red, blue, green), bichromatic (red and blue light combinations at different ratios), and trichromatic (red, blue, and green light combinations at different ratios) lights were applied at the same intensity (100 ± 5 μmol m−2s−1) to enhance cadmium (Cd) accumulation inBidens pilosaL. Compared with the control (fluorescent light), the translocation factor (TF), bioconcentration factor (BCF), and total metal extraction (TE) values of the plant were increased by 13.47–99.24%, 5.33–39.64%, and 20.51–109.62%, respectively, under light-emitting diode (LED) irradiation. The highest BCF and TE values were observed in plants under R20B70G10(20% red, 70% blue, and 10% green light) irradiation, whereas a higher TF value was detected in plants under the condition of R50B50(50% red and 50% blue light) and R20B70G10. Hierarchical clustering and heatmap analysis showed that trichromatic light influenced plant growth, photosynthesis, antioxidant enzyme activity, and soluble protein contents more than bichromatic and monochromatic lights. Additionally, the abundances of rhizosphere microbes and plant growth-promoting rhizobacteria, such asLysobacterandRamlibacter, were significantly higher under LED irradiation, along with enhanced soil enzyme activity. Our findings suggested that the R20B70G10trichromatic light efficiently induces plant Cd extraction and can significantly improve the phytoremediation of Cd-contaminated soils byBidens pilosaL.