Fiber optics fluorescence fingerprint measurement for aerobic plate count prediction on sliced beef surface
Fiber optics fluorescence fingerprint measurement for aerobic plate count prediction on sliced beef surface
复制标题
光纤荧光指纹测量用于预测切片牛肉表面的菌落总数
DOI：
10.1016/j.lwt.2015.11.065
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
J. Sugiyama and Y. Kitamura
中科院分区：
文献类型：
--
作者：
D. M. Mala;M. Yoshimura;S. Kawasaki;M. Tsuta;M. Kokawa;V. Trivittayasil;J. Sugiyama and Y. Kitamura
本文报道了利用荧光指纹（FF）光谱结合光纤技术，通过快速、无损和在线测量来预测肉类表面需氧菌平板计数（APC）的能力。在15 °C下有氧储存3天的牛肉片的FF使用光纤在范围从200 nm至500 nm的激发波长和范围从200 nm至900 nm的发射波长下测量，随后是相同样品的APC。通过偏最小二乘回归（PLSR）测得的FF数据集的决定系数为0.831的APC成功预测。从变量投影重要性（VIP）评分和PLSR获得的回归系数的符号，表明色氨酸，NAD（P）H，维生素A，卟啉和黄素随时间变化，并与预测的APC相关。快速，无损，并在线分析的APC使用FF光谱耦合光纤被证明是适用的。
The ability to predict the aerobic plate count (APC) on a meat surface by rapid, nondestructive, and at-line measurement using fluorescence fingerprint (FF) spectroscopy coupled with fiber optics is reported here. The FF of beef slices stored for 3 days aerobically at 15 °C was measured using fiber optics at excitation wavelengths ranging from 200 nm to 500 nm and emission wavelengths ranging from 200 nm to 900 nm, which was followed by an APC of the same samples. The APC was successfully predicted by partial least squares regression (PLSR) of the measured FF dataset with a coefficient of determination of 0.831. From the variable importance in projection (VIP) score and the sign of the regression coefficient obtained by PLSR, it was indicated that tryptophan, NAD(P)H, Vitamin A, porphyrins, and flavins changed over time and were correlated with predicted APC. Rapid, nondestructive, and at-line analysis of the APC using FF spectroscopy coupled with fiber optics was shown to be applicable.