Identification of some lectin IB4 binding proteins in rat dorsal root ganglia
Identification of some lectin IB4 binding proteins in rat dorsal root ganglia
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DOI：
10.1097/01.wnr.0000136037.54095.64
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发表时间：
2004-08-06
期刊：
影响因子：
1.7
通讯作者：
Elde, R
中科院分区：
文献类型：
--
作者：
Fullmer, JM;Riedl, MS;Elde, R
凝集素是与糖蛋白和糖脂结合的蛋白质。异凝集素Griffonia simplicifolia I-B4（IB 4）识别末端α-半乳糖并结合背根神经节（DRG）中的小型和中型神经元的子集。使用一维和二维凝胶电泳，我们已经确定了几种蛋白质结合IB 4在坐骨神经，背角，和DRG。用酶α-半乳糖苷酶处理降低了IB 4结合，强烈表明结合对IB 4表位是特异性的。α-半乳糖苷酶敏感带的胰蛋白酶解物的质谱分析鉴定了结合IB 4的三种蛋白质：层粘连蛋白β 2链和神经丝的轻亚基和中亚基。
Lectins are proteins that bind to glycoproteins and glycolipids. The isolectin Griffonia simplicifolia I-B4 (IB4) recognizes terminal cc-galactose and binds to a subset of small and medium-sized neurons in the dorsal root ganglia (DRG). Using one and two-dimensional gel electrophoresis, we have identified several proteins that bind IB4 in sciatic nerve, dorsal horn, and DRG. Treatment with the enzyme alpha-galactosidase reduces IB4 binding, strongly suggesting the binding is specific for the IB4 epitope. Mass spectrometric analysis of tryptic digests of alpha-galactosidase sensitive bands identified three proteins that bind IB4: the laminin beta2 chain and the light and medium subunits of neurofilaments.