Isolation and purification of soyasapogenol A and B starting from soy hypocotyls: High-speed counter-current chromatography versus preparative high-performance liquid chromatography
Isolation and purification of soyasapogenol A and B starting from soy hypocotyls: High-speed counter-current chromatography versus preparative high-performance liquid chromatography
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DOI：
10.1080/10826076.2020.1851253
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发表时间：
2020-11-24
影响因子：
1.3
通讯作者：
Zhao, Dayun
中科院分区：
文献类型：
--
作者：
Pan, Chenghui;Qi, Dongyue;Zhao, Dayun
大豆皂甙元是大豆下胚轴中大量存在的一类具有重要生理活性的生物活性物质。然而，缺乏高效和可扩展的制备和分离方法。本研究对高速逆流色谱（HSCCC）和制备型高效液相色谱（prep-HPLC）单独和/或联合应用于大豆皂甙元醇A和B的制备分离进行了研究和比较。结果表明，以大豆胚轴为原料，采用两步HSCCC分离工艺，大豆皂精醇A和B的得率分别为0.24%和0.21%，纯度分别为98.09%和98.90%。相比之下，制备型HPLC允许以显著较低的生产率和较高的溶剂消耗分离和纯化这两种目标化合物。确定了正己烷-乙酸乙酯-甲醇-水（3：6：4：2，v/v/v/v/v）为分离大豆皂草酚的最佳两相溶剂体系。本研究表明，两步高速逆流色谱法可以提供一个快速，高产率，更环保的工具，制备分离和纯化大豆下胚轴的材料开始的大豆皂苷元醇A和B。
Soyasapogenols derived from soyasaponins, which abundantly occur in soy hypocotyls, are of great importance as bioactive compounds due to their physiological activities. However, there is a lack of a high-efficiency and scalable preparation and isolation procedure. This work extends research on applying and comparing high-speed countercurrent chromatography (HSCCC) and preparative high-performance liquid chromatography (prep-HPLC) either singly and/or combinedly in preparative isolation of soyasapogenol A and B. The comparison reveals that the yield of 0.24 and 0.21% of soyasapogenol A and B with the purity of 98.09 and 98.90%, respectively, were obtained from the starting material of soy hypocotyls in two-steps HSCCC separation procedure. In contrast, prep-HPLC allowed isolation and purification of these two target compounds with significantly lower productivity and higher solvent consumption. An optimal two-phase solvent system of n-hexane-ethyl acetate-methanol-water (3:6:4:2, v/v/v/v) was selected to separate soyasapogenols in HSCCC procedure. The present investigation indicates that two-steps HSCCC can potentially provide a fast, high yielding, and more environmentally friendly tool for the preparative separation and purification of soyasapogenol A and B starting from the materials of soy hypocotyls.