Bartonella melophagi in blood of domestic sheep (Ovis aries) and sheep keds (Melophagus ovinus) from the southwestern US: Cultures, genetic characterization, and ecological connections
Bartonella melophagi in blood of domestic sheep (Ovis aries) and sheep keds (Melophagus ovinus) from the southwestern US: Cultures, genetic characterization, and ecological connections
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DOI：
10.1016/j.vetmic.2016.05.009
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发表时间：
2016-07-15
影响因子：
3.3
通讯作者：
Chomel, Bruno
中科院分区：
文献类型：
--
作者：
Kosoy, Michael;Bai, Ying;Chomel, Bruno
甜菜巴尔通体属11月从家养绵羊血液和来自美国西南部的绵羊红(Melophagus Ovinus)中分离得到。用6个分子标记对参考菌株进行的序列分析一致地表明，噬黑假单胞菌与从家畜和野生反刍动物中分离的其他巴尔通体物种相似，但又不同。在与反刍动物相关的其他巴尔通体物种的基因组中不存在的183个针对黑噬杆菌的基因的存在，也支持了该细菌物种与其他反刍动物物种的分离。在所有被调查的绵羊中都检测到了巴尔通体DNA；然而，从绵羊血液中培养这些细菌拒绝了关于噬血巴尔通体是一种强制性内共生菌的推测。相反，这些结果支持这样的假设，即家羊是黑噬杆菌的自然宿主宿主，绵羊是其主要媒介。这种细菌不是从属于同一山羊亚科的大角绵羊和家山羊的血液中分离出来的。噬血杆菌也被证明是人畜共患病，需要进一步调查。爱思唯尔出版公司(Elsevier B.V.)
Bartonella melophagi sp. nov. was isolated from domestic sheep blood and from sheep Reds (Melophagus ovinus) from the southwestern United States. The sequence analyses of the reference strain performed by six molecular markers consistently demonstrated that B. melophagi relates to but differ from other Bartonella species isolated from domestic and wild ruminants. Presence of 183 genes specific for B. melophagi, being absent in genomes of other Bartonella species associated with ruminants also supports the separation of this bacterial species from species of other ruminants. Bartonella DNA was detected in all investigated sheep keds; however, culturing of these bacteria from sheep blood rejects a speculation that B. melophagi is an obligatory endosymbiont. Instead, the results support the hypothesis that the domestic sheep is a natural host reservoir for B. melophagi and the sheep Red its main vector. This bacterium was not isolated from the blood of bighorn sheep and domestic goats belonging to the same subfamily Caprinae. B. melophagi has also been shown to be zoonotic and needs to be investigated further. Published by Elsevier B.V.