A histidine-kinase cheA gene of Pseudomonas pseudoalcaligens KF707 not only has a key role in chemotaxis but also affects biofilm formation and cell metabolism
A histidine-kinase cheA gene of Pseudomonas pseudoalcaligens KF707 not only has a key role in chemotaxis but also affects biofilm formation and cell metabolism
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DOI：
10.1080/08927014.2010.537099
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发表时间：
2011-01
期刊：
影响因子：
2.7
通讯作者：
V. Tremaroli;S. Fedi;S. Tamburini;C. Viti;E. Tatti;H. Ceri;R. Turner;D. Zannoni
中科院分区：
文献类型：
--
作者：
V. Tremaroli;S. Fedi;S. Tamburini;C. Viti;E. Tatti;H. Ceri;R. Turner;D. Zannoni
假产碱假单胞菌KF707中的组氨酸激酶cheA基因在代谢反应的调节以及趋化性中起核心作用。非趋化突变体窝藏插入到cheA基因进行了筛选，他们的能力，在卡尔加里生物膜装置形成生物膜。值得注意的是，与KF707野生型生物膜表型相比，在cheA突变体中观察到附着于聚苯乙烯表面的细胞数量减少≥95%。形成成熟生物膜的能力恢复到野生型水平，为突变体提供了KF 707 cheA基因的功能性拷贝。此外，表型微阵列和蛋白质组学分析显示，几个基本的代谢活动和一些周质结合蛋白的cheA突变细胞与野生型细胞相比，不同。这些结果被解释为在由P.pseudoalcaligenes KF 707的生物膜形成过程中趋化和代谢途径之间的强整合的证据。
A histidine-kinase cheA gene in Pseudomonas pseudoalcaligenes KF707 plays a central role in the regulation of metabolic responses as well as in chemotaxis. Non-chemotactic mutants harboring insertions into the cheA gene were screened for their ability to form biofilms in the Calgary biofilm device. Notably, ≥95% decrease in the number of cells attached to the polystyrene surface was observed in cheA mutants compared to the KF707 wild-type biofilm phenotype. The ability to form mature biofilms was restored to wild-type levels, providing functional copies of the KF707 cheA gene to the mutants. In addition, phenotype micro-arrays and proteomic analyses revealed that several basic metabolic activities and a few periplasmic binding proteins of cheA mutant cells differed compared to those of wild-type cells. These results are interpreted as evidence of a strong integration between chemotactic and metabolic pathways in the process of biofilm development by P. pseudoalcaligenes KF707.