MUC1 is involved in trophoblast transendothelial migration
MUC1 is involved in trophoblast transendothelial migration
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DOI：
10.1016/j.bbamcr.2007.04.006
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发表时间：
2007-06-01
影响因子：
5.1
通讯作者：
Douglas, Gordon C.
中科院分区：
文献类型：
--
作者：
Thirkill, Twanda L.;Cao, Tim;Douglas, Gordon C.
调节滋养层侵入子宫脉管系统的因素尚不完全清楚。在本文中，我们证明猕猴滋养层表达粘蛋白 MUC1，并且它参与滋养层-内皮细胞的相互作用。免疫细胞化学、蛋白质印迹和 RT-PCR 分析证实，分离的早期妊娠猕猴滋养层表达 MUC1。妊娠早期胎盘蜕膜组织切片的血管内滋养层中也检测到了 MUC1。针对 MUC1 的阻断抗体可减少滋养层与子宫内皮细胞的粘附，并阻断滋养层跨内皮细胞迁移。已知 MUC1 在其他系统中与细胞间粘附分子 1 (ICAM-1) 结合。在针对细胞间粘附分子 1 (ICAM-1) 或重组 ICAM-1 的封闭抗体存在下进行孵育，会适度但显着地减少跨内皮滋养层迁移。这些结果与MUC1参与滋养层与子宫内皮细胞的粘附以及滋养层跨内皮细胞迁移的观点一致。 (c) 2007 Elsevier B.V. 保留所有权利。
The factors that regulate trophoblast invasion of the uterine vasculature are incompletely understood. In this paper we show that macaque trophoblasts express the mucin, MUC1, and that it is involved in trophoblast-endothelial interaction. Immunocytochemistry, Western blotting and RT-PCR analyses confirmed that MUC1 was expressed by isolated early gestation macaque trophoblasts. MUC1 was also detected in endovascular trophoblasts in sections of placental-decidual tissue during early gestation. A blocking antibody against MUC1 reduced trophoblast adhesion to uterine endothelial cells and also blocked trophoblast transendothelial migration. MUC1 is known to bind to Intercellular Adhesion Molecule-1 (ICAM-1) in other systems. Incubation in the presence of a blocking antibody against Intercellular Adhesion Molecule-1 (ICAM-1) or recombinant ICAM-1 modestly, but significantly, reduced transendothelial trophoblast migration. These results are consistent with the idea that MUC1 is involved in trophoblast adhesion to uterine endothelial cells and in trophoblast transendothelial migration. (c) 2007 Elsevier B.V. All rights reserved.