Phosphorylation of RAV1/2 by KIN10 is essential for transcriptional activation of CAT6/7, which underlies oxidative stress response in cassava.
Phosphorylation of RAV1/2 by KIN10 is essential for transcriptional activation of CAT6/7, which underlies oxidative stress response in cassava.
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DOI：
10.1016/j.celrep.2021.110119
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发表时间：
2021-12
期刊：
影响因子：
8.8
通讯作者：
Peng Wang;Yu Yan;Yujing Bai;Yabin Dong;Yunxie Wei;Hongqiu Zeng;Haitao Shi
中科院分区：
文献类型：
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作者：
Peng Wang;Yu Yan;Yujing Bai;Yabin Dong;Yunxie Wei;Hongqiu Zeng;Haitao Shi
与ABI 3/VP 1相关的转录因子（RAV）在植物逆境应答中具有重要作用，但RAV是否调控木薯的氧化胁迫应答尚不清楚。在这项研究中，我们报告MeRAV 1/2积极调节木薯的抗氧化胁迫和过氧化氢酶（CAT）活性。同时，RNA测序（RNA-seq）鉴定了MeRAV 1/2沉默的木薯叶片中差异表达的三种MeCAT。有趣的是，MeCAT 6和MeCAT 7被鉴定为MeRAV 1/2通过结合其启动子的直接转录靶点。此外，蛋白激酶MeKIN 10直接与MeRAV 1/2相互作用，分别磷酸化它们的Ser 45和Ser 44残基，以促进它们在MeCAT 6和MeCAT 7上的直接转录激活。MeRAV 1 S45 A或MeRAV 2S 44 A位点突变对MeCAT 6和MeCAT 7启动子活性及抗氧化应激能力无显著影响。总之，本研究表明MeKIN 10对MeRAV 1/2的磷酸化是其在氧化应激反应中直接转录激活MeCAT 6/7所必需的。
Related to ABI3/VP1 (RAV) transcription factors have important roles in plant stress responses; however, it is unclear whether RAVs regulates oxidative stress response in cassava (Manihot esculenta). In this study, we report that MeRAV1/2 positively regulate oxidative stress resistance and catalase (CAT) activity in cassava. Consistently, RNA sequencing (RNA-seq) identifies threeMeCATsthat are differentially expressed inMeRAV1/2-silenced cassava leaves. Interestingly,MeCAT6andMeCAT7are identified as direct transcriptional targets of MeRAV1/2 via binding to their promoters. In addition, protein kinase MeKIN10 directly interacts with MeRAV1/2 to phosphorylate them at Ser45 and Ser44 residues, respectively, to promote their direct transcriptional activation onMeCAT6andMeCAT7. Site mutation of MeRAV1S45Aor MeRAV2S44Ahas no significant effect on the activities ofMeCAT6andMeCAT7promoters or on oxidative stress resistance. In summary, this study demonstrates that the phosphorylation of MeRAV1/2 by MeKIN10 is essential for its direct transcriptional activation of MeCAT6/7in response to oxidative stress.