Visualization of RNA-protein interactions in living cells: FMRP and IMP1 interact on mRNAs
Visualization of RNA-protein interactions in living cells: FMRP and IMP1 interact on mRNAs
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DOI：
10.1038/sj.emboj.7600341
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发表时间：
2004-08-18
期刊：
影响因子：
11.4
通讯作者：
Brown, CM
中科院分区：
文献类型：
--
作者：
Rackham, O;Brown, CM
蛋白质的表达在很大程度上取决于mRNA的稳定性、翻译效率和定位。这些品质在很大程度上取决于与mRNA相关的RNA结合蛋白。在这里，我们报告了一种方法来可视化和本地化RNA蛋白质相互作用在活的哺乳动物细胞。使用这种方法，我们发现，脆性X智力低下蛋白（FMRP）亚型18和人类邮编结合蛋白1直系同源物IMP 1，RNA转运因子，是目前常见的mRNA。这些相互作用主要发生在细胞质中的颗粒状结构。此外，FMRP和IMP1相互作用独立于RNA。FMRP与mRNA的连接导致IMP 1被募集到相同的mRNA，并导致颗粒形成。FMRP和IMP1的密切关联表明哺乳动物细胞中mRNA转运和翻译抑制之间的联系。
Protein expression depends significantly on the stability, translation efficiency and localization of mRNA. These qualities are largely dictated by the RNA-binding proteins associated with an mRNA. Here, we report a method to visualize and localize RNA-protein interactions in living mammalian cells. Using this method, we found that the fragile X mental retardation protein (FMRP) isoform 18 and the human zipcode-binding protein 1 ortholog IMP1, an RNA transport factor, were present on common mRNAs. These interactions occurred predominantly in the cytoplasm, in granular structures. In addition, FMRP and IMP1 interacted independently of RNA. Tethering of FMRP to an mRNA caused IMP1 to be recruited to the same mRNA and resulted in granule formation. The intimate association of FMRP and IMP1 suggests a link between mRNA transport and translational repression in mammalian cells.