ENZYME-LINKED IMMUNOASSAY FOR ANTI-HLA ANTIBODIES - AN ALTERNATIVE TO PANEL STUDIES BY LYMPHOCYTOTOXICITY
ENZYME-LINKED IMMUNOASSAY FOR ANTI-HLA ANTIBODIES - AN ALTERNATIVE TO PANEL STUDIES BY LYMPHOCYTOTOXICITY
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DOI：
10.1097/00007890-199301000-00036
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发表时间：
1993-01-01
期刊：
影响因子：
6.2
通讯作者：
SMALL, SJ
中科院分区：
文献类型：
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作者：
KAO, KJ;SCORNIK, JC;SMALL, SJ
为了提供一种简便、特异的方法来检测和定量检测血清中的抗人类白细胞抗原抗体，从240例随机献血者的血小板中分离纯化的人类白细胞抗原，建立了固相酶联免疫分析方法。通过对39例未进行过同种异体免疫的健康人血清进行检测，确定了判断是否存在抗-HLAIg或Ig M抗体的参考值。通过研究122名患者的血清，对该方法进行了评估，这些患者以前曾被淋巴细胞毒试验(LCA)鉴定为群体反应性抗体。两种方法之间存在显著的线性相关(r=0.80，P=0.0001)。进一步的数据分析表明，与LCA相比，新开发的EIA具有100%的特异度和95.3%的灵敏度。其他研究表明，PRA随时间增加或减少的患者与EIA测量的抗体水平平行。EIA法检测抗-HLA抗体效价较LCA法灵敏。由于EIA的敏感性、特异性和技术要求较低，它应该提供一个有用的替代方案，以减少监测器官移植候选者和输血接受者抗人类白细胞抗原抗体状况的更费力的小组研究的数量。
In order to provide a simple and specific assay for the detection and quantitation of IgG and IgM anti-HLA antibodies in sera, HLA antigens purified from a pool of 240 random donor platelets were used to develop a solid-phase enzyme-linked immunoassay (EIA). The reference values for identifying the presence of IgG or IgM anti-HLA antibodies were determined by assaying sera from 39 healthy individuals without prior HLA alloimmunization. The assay was evaluated by studying sera from 122 patients who had been characterized previously for panel reactive antibodies by the lymphocytotoxicity assay (LCA). A significant linear correlation between two assays was noted (r=0.8, P=0.0001). Further analyses of the data demonstrated that the newly developed EIA has 100% specificity and 95.3% sensitivity as compared with the LCA. Additional studies revealed that patients whose PRA increased or decreased over time were in parallel with antibody levels measured by EIA. When the EIA was used to measure anti-HLA antibody titers, it was more sensitive than the LCA. Since the EIA is sensitive, specific, and technically less demanding, it should provide an useful alternative to reduce the number of the more laborious panel studies for monitoring anti-HLA antibody status in candidates for organ transplantation and recipients of blood transfusions.